Abstract
Recently E-selectin (ELAM-1, endothelial leukocyte adhesion molecule-1) was shown to recognize not only sialyl Lewis X but also sialyl Lewis A, and these carbohydrate antigens may be involved in the process of the adhesion between cancer cells and endothelial cells in cancer metastasis. To investigate the contribution of sialylated carbohydrate antigen, SPan-1, and sialic acid to the adhesion of human colon cancer cells to endothelial cells, adhesion assay using HUVECs (human umbilical vein endothelial cells) was performed. The adhesion was significantly inhibited by pretreatment with anti-E-selectin antibody, indicating that this adhesion was thought to be mediated by E-selectin. When these cancer cells were pretreated with SPan-1 antibody, the adhesion was significantly inhibited in a concentration-dependent manner. The adhesion was also inhibited by pretreatment with neuraminidase. These findings suggest that the SPan-1 antigen plays a significant role in the adhesion of human colon cancer cells to endothelial cells, and sialylation of the terminal structure of carbohydrate antigens is important in this adhesion.
References
Nov 2, 1990·Cell·J B LoweR M Marks
Nov 23, 1990·Science·M L PhillipsJ C Paulson
Nov 23, 1990·Science·G WalzB Seed
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M TiemeyerB K Brandley
Sep 16, 1991·Biochemical and Biophysical Research Communications·A TakadaR Kannagi
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D TyrrellD Asa
Nov 1, 1991·Japanese Journal of Cancer Research : Gann·K TakedaK Kumagai
Jan 1, 1986·Cancer Investigation·S C LottichJ Schlom
Jan 1, 1985·Advances in Cancer Research·V Schirrmacher
Oct 15, 1985·International Journal of Cancer. Journal International Du Cancer·M KawaharaT Iguro
Jan 1, 1987·Cancer Metastasis Reviews·A Raz, R Lotan
Oct 1, 1987·Cancer·Y S ChungK Umeyama
Jun 3, 1985·European Journal of Biochemistry·F G HanischE Hippauf
Jan 1, 1984·Biochimica Et Biophysica Acta·E Roos
Jan 1, 1983·Advances in Cancer Research·G Yogeeswaran
Jun 26, 1981·Science·G Yogeeswaran, P L Salk
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Citations
Apr 10, 2013·World Journal of Surgery·Naoshi KuboKosei Hirakawa
Mar 18, 2009·Medicinal Research Reviews·Shan JinBinghe Wang
Nov 22, 2007·Expert Opinion on Therapeutic Targets·Steven R BarthelCharles J Dimitroff
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Miranda ten KateCasper H J van Eijck
May 23, 2006·Oncogene·P-L TremblayJ Huot
Jul 3, 2008·Cancer Research·Pierre-Luc TremblayFrançois A Auger