Role of spastin and protrudin in neurite outgrowth

Journal of Cellular Biochemistry
Chuanling ZhangXu Ma

Abstract

Hereditary spastic paraplegia (HSP) is a neurodegenerative disorder characterized by retrograde axonal degeneration that primarily affects long spinal neurons. The gene encoding spastin has a well-established association with HSP, and protrudin is a known binding partner of spastin. Here, we demonstrate that the N-terminal domain of protrudin mediates the interaction with spastin, which is responsible for neurite outgrowth. We show that spastin promotes protrudin-dependent neurite outgrowth in PC12 cells. To further confirm these physiological functions in vivo, we microinjected zebrafish embryos with various protrudin/spastin mRNA and morpholinos. The results suggest that the spinal cord motor neuron axon outgrowth of zebrafish is regulated by the interaction between spastin and protrudin. In addition, the putative HSP-associated protrudinG191V mutation was shown to alter the subcellular distribution and impair the yolk sac extension of zebrafish, but without significant defects in neurite outgrowth both in PC12 cells and zebrafish. Taken together, our findings indicate that protrudin interacts with spastin and induces axon formation through its N-terminal domain. Moreover, protrudin and spastin may work together to play an in...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jun 8, 1999·Chemistry and Physics of Lipids·J M GaullierH Stenmark
Aug 10, 2000·Journal of Neurobiology·K KalilE W Dent
Mar 26, 2002·FEBS Letters·Harald StenmarkPaul C Driscoll
May 22, 2002·Archives of Neurology·Peter HederaJohn K Fink
Nov 5, 2003·Journal of Neurobiology·Phillip R Gordon-Weeks
Feb 18, 2005·The Journal of Cell Biology·Katia J EvansBrett P Lauring
Oct 13, 2005·Journal of Neurochemistry·Sara SalinasThomas T Warner
Apr 29, 2006·Traffic·Peter W BaasKenneth A Myers
Jun 7, 2006·IUBMB Life·Tadaomi Takenawa, Toshiki Itoh
Jul 11, 2006·American Journal of Human Genetics·Ashraf U MannanWolfgang Engel
Aug 9, 2006·Human Molecular Genetics·Jonathan D WoodVincent T Cunliffe
Nov 4, 2006·Science·Michiko Shirane, Keiichi I Nakayama
Nov 10, 2007·Current Opinion in Neurology·Christel DepienneAlexandra Durr
May 8, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Michiko ShiraneKeiichi I Nakayama
Jun 11, 2008·Current Neurology and Neuroscience Reports·Giovanni StevaninAlexis Brice
Jul 9, 2008·American Journal of Human Genetics·Monica MartignoniElena I Rugarli
Nov 15, 2008·Lancet Neurology·Sara SalinasThomas T Warner
Jun 10, 2010·The Journal of Cell Biology·Benjamin LacroixCarsten Janke
Sep 6, 2011·Biochimica Et Biophysica Acta·Jennifer H LumbEvan Reid

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Citations

Aug 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jaerak ChangCraig Blackstone
Jun 26, 2015·The European Journal of Neuroscience·Tijana Copf
Apr 8, 2014·Progress in Neurobiology·Patrick J BabinDemetrio Raldúa
Nov 28, 2019·Cell Death & Disease·Xi LuXuefeng Wang
Nov 17, 2020·Frontiers in Cellular Neuroscience·Tao JiangMinghui Tan
Jan 2, 2021·Frontiers in Cell and Developmental Biology·Michiko Shirane

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