Role of spinal Cav2.2 and Cav2.1 ion channels in bladder nociception

The Journal of Urology
Xin SuNicholas J Laping

Abstract

High voltage activated calcium channels have been implicated in nociceptive transmission in several animal pain models. To our knowledge this is the first study to evaluate the ability of various high voltage activated calcium channel blockers to inhibit the transmission of noxious stimuli from the bladder at the level of the spinal cord. The nociceptive response was measured by analyzing the visceromotor reflex and cardiovascular (pressor) responses to bladder distention. The role of Cav2.2 (N-type), Cav2.1 (P/Q-type) and Cav1 (L-type) calcium channels in bladder nociceptive reflex responses was examined using omega-conotoxin-GVIA, omega-agatoxin IVA/omega-conotoxin MVIIC and verapamil (Sigma-Aldrich), respectively. Female Sprague-Dawley rats were acutely instrumented with intrathecal catheters, carotid arterial and bladder cannulas. Needle electrodes were placed directly into the abdominal musculature to measure myoelectric activity subsequent to repeat phasic bladder distention at 60 mm Hg for 30 seconds at 3-minute intervals with the rats under 1% isoflurane. Drugs were administered by intrathecal injection 2 minutes before distention and responses were recorded for 15 minutes per dose. When administered intrathecally, omeg...Continue Reading

References

May 1, 1990·Pain·T J Ness, G F Gebhart
Aug 12, 2000·The Journal of Biological Chemistry·R J LewisP F Alewood
Jan 5, 2002·The Journal of Biological Chemistry·Taiji TsunemiTsutomu Tanabe
Nov 7, 2006·Pain·Yu-Qing Cao
Jun 29, 2007·Neurourology and Urodynamics·Xin SuNicholas J Laping

❮ Previous
Next ❯

Citations

Jul 24, 2012·Marine Drugs·Magbubah EssackJohn A C Archer
Dec 15, 2010·Channels·John Park, Z David Luo
Jul 26, 2011·Pain·Tony L Yaksh
Jun 20, 2008·American Journal of Physiology. Renal Physiology·Xin SuNicholas J Laping
Jul 18, 2008·American Journal of Physiology. Renal Physiology·Xin SuNicholas J Laping
Aug 3, 2018·Biological & Pharmaceutical Bulletin·Fumiko SekiguchiAtsufumi Kawabata
Jun 20, 2008·American Journal of Physiology. Renal Physiology·Erin S R LashingerXin Su
Feb 29, 2020·Current Neuropharmacology·Xuechao HaoCheng Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.