Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation.

Journal of Bacteriology
Hector Benito de la PueblaAimee Shen

Abstract

The nosocomial pathogen Clostridioides difficile is a spore-forming obligate anaerobe that depends on its aerotolerant spore form to transmit infections. Functional spore formation depends on the assembly of a proteinaceous layer known as the coat around the developing spore. In C. difficile, coat assembly depends on the conserved spore protein SpoIVA and the clostridial-organism-specific spore protein SipL, which directly interact. Mutations that disrupt their interaction cause the coat to mislocalize and impair spore formation. In Bacillus subtilis, SpoIVA is an ATPase that uses ATP hydrolysis to drive its polymerization around the forespore. Loss of SpoIVA ATPase activity impairs B. subtilis SpoIVA encasement of the forespore and activates a quality control mechanism that eliminates these defective cells. Since this mechanism is lacking in C. difficile, we tested whether mutations in the C. difficile SpoIVA ATPase motifs impact functional spore formation. Disrupting C. difficile SpoIVA ATPase motifs resulted in phenotypes that were typically >104-fold less severe than the equivalent mutations in B. subtilis Interestingly, mutation of ATPase motif residues predicted to abrogate SpoIVA binding to ATP decreased the SpoIVA-SipL ...Continue Reading

References

Feb 1, 1995·Microbiology·T KarasawaS Nakamura
Aug 1, 1993·Molecular Microbiology·P A LevinS Cutting
Jun 1, 1993·Journal of Bacteriology·K SmithP Youngman
Apr 1, 1997·Molecular Microbiology·J A Londoño-VallejoP Stragier
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Apr 12, 2007·Molecular Microbiology·Kumaran S RamamurthiRichard Losick
Nov 24, 2007·Annual Review of Microbiology·Adriano O Henriques, Charles P Moran
Aug 12, 2008·Molecular Cell·Kumaran S Ramamurthi, Richard Losick
Feb 24, 2009·Current Protocols in Microbiology·Joseph A Sorg, Sean S Dineen
Mar 7, 2009·Science·Kumaran S RamamurthiRichard Losick
Sep 25, 2009·Molecular Microbiology·Katherine H WangPatrick Eichenberger
Dec 17, 2011·Molecular Microbiology·Peter T McKenney, Patrick Eichenberger
Apr 3, 2012·Molecular Microbiology·Sarah E EbmeierKumaran S Ramamurthi
May 23, 2012·Infection and Immunity·Laura J DeakinTrevor D Lawley
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe CastaingKumaran S Ramamurthi
Jan 8, 2013·Journal of Bacteriology·Emily E PutnamAimee Shen
Feb 12, 2013·Journal of Bacteriology·Ana B AbecasisAdriano O Henriques
Jul 10, 2013·Environmental Microbiology·Natalya Yutin, Michael Y Galperin
Aug 21, 2013·PLoS Genetics·Kelly A FimlaidAimee Shen
Jan 30, 2014·PloS One·Andrea DegasperiBoris N Kholodenko
May 13, 2014·Trends in Microbiology·Daniel Paredes-SabjaJoseph A Sorg
Jul 2, 2014·Environmental Microbiology Reports·Irene S Tan, Kumaran S Ramamurthi
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Richard L GillKumaran S Ramamurthi
Sep 22, 2015·Developmental Cell·Irene S TanKumaran S Ramamurthi
Oct 22, 2015·Annual Review of Microbiology·Casey M Theriot, Vincent B Young
May 10, 2016·Nature Reviews. Disease Primers·Wiep Klaas SmitsEd J Kuijper
May 27, 2016·Microbiology Spectrum·Adam Driks, Patrick Eichenberger
Jun 24, 2016·Microbiology Spectrum·David L Popham, Casey B Bernhards
Aug 11, 2016·Methods in Molecular Biology·Aimee ShenKeyan Pishdadian
Aug 31, 2016·Nature Reviews. Microbiology·Michael C AbtEric G Pamer
Oct 12, 2016·Microbiology Spectrum·Michelle C SwickAdam Driks
Apr 15, 2017·Trends in Microbiology·Amanda R Decker, Kumaran S Ramamurthi
Jun 16, 2017·Annual Review of Microbiology·Brittany B Lewis, Eric G Pamer
Oct 3, 2017·The Journal of Infectious Diseases·Huynh A HongSimon M Cutting
Oct 7, 2017·Frontiers in Microbiology·Marcin DembekSimon M Cutting
Oct 20, 2017·FEMS Microbiology Reviews·Ramyavardhanee Chandrasekaran, D Borden Lacy

❮ Previous
Next ❯

Citations

Mar 27, 2021·Frontiers in Microbiology·Polina BeskrovnayaElitza I Tocheva

❮ Previous
Next ❯

Methods Mentioned

BETA
coaffinity purification
GTPases
spore
transmission electron microscopy
two-hybrid
coimmunoprecipitation
immunoprecipitations
pulldown
two hybrid
PCR

Software Mentioned

Adobe Photoshop CC
SpoVM
NIS
Elements
Genewiz

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.