Role of staphylococcal superantigen in atopic dermatitis: influence on keratinocytes.

Journal of Korean Medical Science
Kyu Han KimHee Chul Eun

Abstract

Staphylococcus aureus may perform an crucial function in atopic dermatitis (AD), via the secretion of superantigens, including staphylococcal enterotoxins (SE) A or B, and toxic shock syndrome toxin-1 (TSST-1). Dysregulated cytokine production by keratinocytes (KCs) upon exposure to staphylococcal superantigens (SsAgs) may be principally involved in the pathophysiology of AD. We hypothesized that lesional KCs from AD may react differently to SsAgs compared to nonlesional skin or normal skin from nonatopics. We conducted a comparison of HLA-DR or CD1a expression in lesional skin as opposed to that in nonlesional or normal skin by immunohistochemistry (IHC). We also compared, using ELISA, the levels of IL-1alpha, IL-1beta, and TNF-alpha secreted by cultured KCs from lesional, nonlesional, and normal skin, after the addition of SEA, SEB and TSST-1. IHC revealed that both HLA-DR and CD1a expression increased significantly in the epidermis of lesional skin versus nonlesional or normal skin in quite a similar manner. IL-1alpha, IL-1beta, and TNF-alpha secretion was also significantly elevated in the cultured KCs from lesional skin after the addition of SsAgs. Our results indicated that KCs from lesional skin appear to react different...Continue Reading

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Citations

Mar 25, 2014·The Journal of Allergy and Clinical Immunology·Ting Seng TangHywel C Williams
May 30, 2009·Advances in Immunology·Michiko K OyoshiRaif S Geha
Oct 22, 2016·The Journal of Investigative Dermatology·Michael R WilliamsRichard L Gallo
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Sep 18, 2007·Current Opinion in Allergy and Clinical Immunology·Sonja Bonness, Thomas Bieber
Oct 14, 2021·Investigative Ophthalmology & Visual Science·Hua ChenMing-Chang Zhang

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
PCR

Software Mentioned

Raytest

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Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.