Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells

ACS Omega
Thangavel MuthukumarasamyvelNagappan Rajendiran

Abstract

Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The haemocompatibility of the synthesized NPs was evaluated by in vitro hemolysis and erythrocyte sedimentation rate using human red blood cells (RBCs). In all of the systems, no toxicity was observed on human erythrocytes (RBCs) up to the concentration of 120 μg/mL. The anticancer activity of these dicationic amphiphile-stabilized AuNPs on A549 lung cancer cells was demonstrated by in vitro cell viability assay, intracellular reactive oxygen species estimation by DCFH-DA, apoptosis analysis using AO-EtBr fluorescence staining, DNA fragmentation analysis by agarose gel electrophoresis, and western blot analysis of caspase-3 expression. These results suggest that the cytotoxicity of AuNPs to A549 cells increase with the dose and hydrophobicity of amphiphiles and were found to be in the order: DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs.

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Citations

May 14, 2020·Journal of Cellular Physiology·Yadong WangHaiyan Yang
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Hainan SunShumei Zhai
Mar 17, 2020·Drug Delivery and Translational Research·Arpit BhargavaPradyumna Kumar Mishra
Mar 10, 2018·International Journal of Molecular Sciences·Hainan SunShumei Zhai
Oct 1, 2018·Nanoscale Research Letters·Mei-Xia ZhaoZhi-Qiang Zhang
Mar 15, 2020·Nanomaterials·Hainan SunShumei Zhai

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Methods Mentioned

BETA
surface plasmon resonance
transmission electron microscopy
dynamic light scattering
ESR
light microscopy
inverted microscopy
Fluorescence Microscopy
DNA shearing
electrophoresis

Software Mentioned

SPSS
Image
imagej

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