Abstract
This study was designed to assess human bone marrow cell response and particularly cell adhesion, proliferation, and differentiation, when cultured in vitro on titanium alloy and hydroxyapatite with different values of surface roughness. A further aim was to compare the cell response on the two materials, currently used in spinal surgery. Cell adhesion was determined after 0.5, 2, 4, and 18 hours of incubation; proliferation after 8, 11, 14, and 16 days of culture; and differentiation was evaluated with the expression of alkaline phosphatase activity after 8 and 16 days of culture. This study showed that bone marrow cells grew faster on titanium alloy than on hydroxyapatite, although fewer cells attached to titanium, compared to those attached to hydroxyapatite. No statistically significant difference was observed as the expression of alkaline phosphatase activity on hydroxyapatite and titanium alloy of the same roughness. Cell adhesion, proliferation, and differentiation are dependent on surface roughness of the biomaterial, and all three increased as the roughness of titanium alloy increased. Conclusively, surface roughness of titanium and hydroxyapatite significantly influences bone marrow cell response, and therefore these ...Continue Reading
References
Jun 1, 1991·Journal of Biomedical Materials Research·D A PuleoR Bizios
Jul 1, 1991·Journal of Biomedical Materials Research·D BuserH Stich
Feb 1, 1991·Journal of Periodontology·T G Donley, W B Gillette
Sep 1, 1990·Journal of Biomedical Materials Research·B ChehroudiD M Brunette
Oct 1, 1990·Journal of Biomedical Materials Research·J E DaviesA Shiga
Nov 1, 1989·Journal of Biomedical Materials Research·U E PazzagliaP Cherubino
Sep 1, 1989·Journal of Biomedical Materials Research·B ChehroudiD M Brunette
Oct 1, 1988·Journal of Biomedical Materials Research·B R McAuslanJ G Steele
Jan 1, 1987·Journal of Biomedical Materials Research·T InoueA H Melcher
Aug 1, 1970·Archives of Oral Biology·G H Nancollas, M S Mohan
Mar 1, 1995·Journal of Biomedical Materials Research·J Y MartinB D Boyan
Mar 1, 1995·Biomaterials·G Zambonin, M Grano
Feb 1, 1995·Journal of Biomedical Materials Research·J L OngL C Lucas
Mar 1, 1995·Biomaterials·A HunterG W Blunn
Sep 1, 1993·In Vitro Cellular & Developmental Biology. Animal·J Vilamitjana-AmedeeM F Harmand
May 1, 1996·Biomaterials·K BordjiM Hage-Ali
Jan 1, 1996·Biomaterials·S Ozawa, S Kasugai
Jan 1, 1996·Implant Dentistry·J L OngL C Lucas
Jan 4, 1998·Journal of Biomedical Materials Research·M HottP J Marie
Jan 1, 1997·Dental Materials : Official Publication of the Academy of Dental Materials·J C KellerT D McGee
Apr 29, 1998·Journal of Biomedical Materials Research·A WilkeR P Franke
Apr 29, 1998·Clinical Oral Implants Research·P R KlokkevoldA Caputo
Citations
Dec 25, 2007·Journal of Materials Science. Materials in Medicine·David M PickupRobert J Newport
Apr 10, 2012·Journal of Materials Science. Materials in Medicine·Petros A KokkinosDespina D Deligianni
Jan 24, 2004·Biomaterials·Jin Woo LeeSoo Bong Hahn
Mar 17, 2004·Biomaterials·Eui Kyun ParkSukyoung Kim
Mar 17, 2005·Biomaterials·Veronica BorsariRoberto Giardino
Jun 24, 2003·Biomaterials·Jongsik ChoiMatthias Epple
Aug 16, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Veronica BorsariRoberto Giardino
Aug 5, 2004·Journal of Biomedical Materials Research. Part a·Robert M LevenDale R Sumner
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·John D JarrellJeffrey R Morgan
Jan 11, 2007·Journal of Biomedical Materials Research. Part a·James E HoKevin E Healy
Mar 30, 2012·Birth Defects Research. Part C, Embryo Today : Reviews·Nianli Zhang, David H Kohn
Aug 28, 2010·Macromolecular Bioscience·Vitor M CorreloRui L Reis
Mar 27, 2009·Journal of Biomedical Materials Research. Part a·K HayashiM Naito
Jan 11, 2014·Journal of Biomedical Materials Research. Part a·Krzysztof MaryczAgnieszka Śmieszek
Nov 30, 2004·Biomolecular Engineering·Her-Hsiung HuangFang-Lung Chen
Apr 28, 2004·Biomaterials·J HarrisonR Mollard
Jun 20, 2006·Colloids and Surfaces. B, Biointerfaces·A Méndez-VilasM L González-Martín
Sep 27, 2005·Micron : the International Research and Review Journal for Microscopy·Franchi MarcoOttani Vittoria
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Alieh AminianKurosch Rezwan
Jan 24, 2016·Journal of Biomaterials Applications·Krzysztof MaryczKarolina Moszak
Dec 8, 2016·Journal of Biomechanical Engineering·Kaushik Mukherjee, Sanjay Gupta
Jun 24, 2021·Expert Review of Medical Devices·Jessee P HuntJon E Block
Jul 3, 2021·Materials·Thomas KrellerRainer Detsch