Role of surfactants in optimizing fluorene assimilation and intermediate formation by Rhodococcus rhodochrous VKM B-2469

Bioresource Technology
Marina P KolomytsevaLudmila A Golovleva

Abstract

Biodegradation of fluorene by Rhodococcus rhodochrous VKM B-2469 was investigated and optimized by adding non-ionic surfactants to the liquid media. The utilization of 1-1.5% Tween 60 or 1% Triton X100 allowed to solubilize 1 mM fluorene over 150 times more than in water medium (from 9-11 microM to above 1.5 mM at 28 degrees C). We observed that Tween 60 was useful to enhance the fluorene biodegradation rates further supporting R. rhodochrous VKM B-2469 growth as an additional carbon source and to decrease fluorene toxicity for bacterial cells whereas Triton X100 resulted to be toxic for this strain. An additional enzyme induction step before starting the bioconversion process and the increase of incubation temperature during fluorene bioconversion led to further improvements in rates of fluorene utilization and formation of its intermediates. In the optimized conditions 1 mM fluorene was degraded completely within 24h of incubation. Some intermediates in fluorene degradation built up during the process reaching maxima of 31% for 9-hydroxyfluorene, 2.1% for 9-fluorenone and 1.9% for 2-hydroxy-9-fluorenone (starting from 1 mM substrate). In the presence of Tween 60 the appearance and following conversion of 2-hydroxy-9-fluorenon...Continue Reading

References

Mar 29, 2000·Journal of Bacteriology·R A Kanaly, S Harayama
Dec 17, 2002·The Journal of General and Applied Microbiology·Hideaki NojiriToshio Omori
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Hiroshi Habe, Toshio Omori
Dec 25, 2007·Applied and Environmental Microbiology·Luc SchulerSpiros N Agathos

❮ Previous
Next ❯

Citations

Jul 14, 2010·Environmental Science and Pollution Research International·F Fernández-LuqueñoL Dendooven
Jul 1, 2010·Environmental Science & Technology·Hongbo Zhu, Michael D Aitken
Dec 1, 2015·Journal of Agricultural and Food Chemistry·Jiayuan ZhaoKai Yao
Jul 16, 2010·Bioresource Technology·M Srikanth ReddyPrathibha Devi
Feb 6, 2018·Applied Biochemistry and Biotechnology·Jingjing LiuMin Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.