PMID: 6977083Jan 1, 1981Paper

Role of T cells in the mitogen-induced proliferation and polyclonal antibody response of murine B cells

Microbiology and Immunology
H Ishikawa, K Saito

Abstract

The effect of thymus-derived lymphocytes (T cells) on the responsiveness of bone marrow-derived lymphocytes (B cells) to bacterial lipopolysaccharide (LPS) was determined in in vitro experiments. Radiation resistant splenic T cells obtained from euthymic nu/+ mice increased the number of proliferating cells in the cultures of splenic B cells from athymic nu/nu mice even in the nonstimulated state. The radiation resistant T cells augmented significantly the responsiveness of B cells to LPS, as determined by an increase in proliferating cells and polyclonally induced anti-sheep erythrocyte (SRBC) IgM hemolysin plaque-forming cells (PFC). Addition of the T cells to B cell cultures not only augmented the responsiveness of B cells to suboptimal doses of LPS but also enabled B cells to respond to supraoptimal doses of LPS. As is well documented, the radiation resistant T cells were unable to induce the generation of anti-SRBC PFC in B cell cultures, unless the cultures were simultaneously stimulated with SRBC. Colcemid, a specific inhibitor of cell mitosis, blocked almost completely the exponential generation of anti-SRBC PFC in B cell cultures responding to SRBC with the aid of radiation resistant T cells. In contrast, colcemid did ...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E TzehovalM Feldman
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Jan 1, 1969·Transplantation Reviews·J F Miller, G F Mitchell

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Citations

Sep 21, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luciana BesedovskyTanja Lange

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