PMID: 9420241Jan 7, 1998Paper

Role of the adenovirus DNA-binding protein in in vitro adeno-associated virus DNA replication

Journal of Virology
P WardK I Berns

Abstract

A basic question in adeno-associated virus (AAV) biology has been whether adenovirus (Ad) infection provided any function which directly promoted replication of AAV DNA. Previously in vitro assays for AAV DNA replication, using linear duplex AAV DNA as the template, uninfected or Ad-infected HeLa cell extracts, and exogenous AAV Rep protein, demonstrated that Ad infection provides a direct helper effect for AAV DNA replication. It was shown that the nature of this helper effect was to increase the processivity of AAV DNA replication. Left unanswered was the question of whether this effect was the result of cellular factors whose activity was enhanced by Ad infection or was the result of direct participation of Ad proteins in AAV DNA replication. In this report, we show that in the in vitro assay, enhancement of processivity occurs with the addition of either the Ad DNA-binding protein (Ad-DBP) or the human single-stranded DNA-binding protein (replication protein A [RPA]). Clearly Ad-DBP is present after Ad infection but not before, whereas the cellular level of RPA is not apparently affected by Ad infection. However, we have not measured possible modifications of RPA which might occur after Ad infection and affect AAV DNA repli...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G HongK I Berns
Apr 20, 1991·Journal of Molecular Biology·P Ward, K I Berns
Sep 1, 1990·Microbiological Reviews·K I Berns
Mar 1, 1989·Journal of Virology·B YakobsonE Winocour
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K NagataJ Hurwitz
Nov 1, 1982·Journal of Virology·R A McPhersonJ A Rose
Jan 1, 1995·Current Topics in Microbiology and Immunology·P C Van der Vliet
Jan 1, 1995·Current Topics in Microbiology and Immunology·R T HayA Webster
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·D W RussellA D Miller
Mar 11, 1994·Nucleic Acids Research·A MonaghanR T Hay
Feb 1, 1994·Journal of Virology·T H NiN Muzyczka
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R M LindenK I Berns

❮ Previous
Next ❯

Citations

Nov 3, 2014·Annual Review of Virology·R Jude Samulski, Nicholas Muzyczka
Feb 6, 2020·Viruses·Matthew Charman, Matthew D Weitzman
Jan 8, 2011·Future Virology·Els Henckaerts, R Michael Linden
Apr 19, 2020·Human Gene Therapy·Anna C Maurer, Matthew D Weitzman
May 3, 2011·Human Molecular Genetics·Robert M Kotin
Mar 16, 2007·Journal of Virology·Kevin NashNicholas Muzyczka
Mar 30, 2000·The Journal of Gene Medicine·R O Snyder
Oct 31, 2008·Journal of Virology·Kevin NashNicholas Muzyczka
Jun 25, 2020·Viruses·Anita F MeierMichael Seyffert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.