Role of the amino-terminal region of streptokinase in the generation of a fully functional plasminogen activator complex probed with synthetic peptides

Protein Science : a Publication of the Protein Society
D NihalaniG Sahni

Abstract

The mechanism whereby fragments of streptokinase (SK) derived from its N terminus (e.g., SK1-59 or SK1-63) enhance the low plasminogen (PG)-activating ability of other fragments, namely SK64-386, SK60-414, SK60-387, and SK60-333 (reported previously), has been investigated using a synthetic peptide approach. The addition of either natural SK1-59, or chemically synthesized SK16-59, at saturation (about 500-fold molar excess) generated amidolytic and PG activation capabilities in equimolar mixtures of human plasminogen (HPG) and its complementary fragment (either SK60-414 or SK56-414, prepared by expression of truncated SK gene fragments in Escherichia coli) that were approximately 1.2- and 2.5-fold, respectively, of that generated by equimolar mixtures of native SK and HPG. Although in the absence of SK1-59 equimolar mixtures of SK56-414 and HPG could generate almost 80% of amidolytic activity, albeit slowly, less than 2% level of PG activation could be observed under the same conditions, indicating that the contribution of the N-terminal region lay mainly in imparting in SK56-414 an enhanced ability for PG activation. The ability of various synthetic peptides derived from the amino-terminal region (SK16-51, SK16-45, SK37-59, SK...Continue Reading

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Citations

Jul 3, 2013·Applied Microbiology and Biotechnology·Essam Kotb
Nov 18, 2006·Journal of Thrombosis and Thrombolysis·Adinarayana KunamneniPoluri Ellaiah
Dec 31, 2003·Biotechnology Advances·Anirban BanerjeeU C Banerjee
Dec 31, 2002·Journal of Immunological Methods·Behnaz Parhami-SerenPanayota Tsantili
Aug 16, 2002·European Journal of Biochemistry·Ana I AzuagaFrancisco Conejero-Lara
Feb 27, 1999·Protein Science : a Publication of the Protein Society·A I AzuagaC M Dobson
May 12, 2009·Bioresource Technology·Deepika GoyalDebendra K Sahoo
Nov 29, 2002·Protein Engineering·N WakehamX C Zhang
Apr 22, 2003·The Journal of Biological Chemistry·Lakshmi V MundadaWilliam P Fay
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Akram HasanpourAmir Amani
Dec 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Deepika GoyalGirish Sahni

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