Role of the Arf6 GDP/GTP cycle and Arf6 GTPase-activating proteins in actin remodeling and intracellular transport

The Journal of Biological Chemistry
Stéphanie KleinFrederic Luton

Abstract

We have analyzed both biochemically and functionally a series of Arf6 mutants, providing new insights into the molecular mode of action of the small G protein Arf6. First, by comparing a fast-cycling mutant (Arf6(T157N)) and a GTPase-deficient mutant (Arf6(Q67L)), we established the necessity for completion of the Arf6 GDP/GTP cycle for recycling of major histocompatibility complex molecules to the plasma membrane. Second, we found that aluminum fluoride (AlF), known for inducing membrane protrusion in cells expressing exogenous wild-type Arf6, stabilized a functional wild-type Arf6.AlF(x) . GTPase-activating protein (GAP) complex in vitro and in vivo. We also found that the tandem mutation Q37E/S38I prevented the binding of two Arf GAPs, but not the effector ARHGAP10, and blocked the formation of membrane protrusion and actin reorganization. Together, our results with AlF(x) and Arf6(Q37E/S38I) demonstrate the critical role of the Arf6 GAPs as effectors for Arf6-regulated actin cytoskeleton remodeling. Finally, competition experiments conducted in vivo suggest the existence of a membrane receptor for GDP-bound Arf6.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J DuronioJ I Gordon
Jan 6, 1995·The Journal of Biological Chemistry·G F ZhangM Vaughan
Feb 24, 1995·Science·C D'Souza-SchoreyP D Stahl
Feb 1, 1997·Cellular Signalling·B TocqueF Schweighoffer
Oct 6, 1997·The Journal of Cell Biology·H Radhakrishna, J G Donaldson
Nov 22, 1997·Current Biology : CB·R LinD Manor
Aug 11, 1998·Trends in Biochemical Sciences·K ScheffzekA Wittinghofer
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M FrancoP Chavrier
Jul 3, 1999·The Journal of Biological Chemistry·J Gaschet, V W Hsu
Aug 7, 1999·The Journal of Biological Chemistry·R LinD Manor
Aug 17, 1999·Current Opinion in Cell Biology·P Chavrier, B Goud
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P A RandazzoJ A Cooper
Jul 6, 2000·Nature Structural Biology·J MénétreyJ Cherfils
Aug 10, 2000·Nature Cell Biology·A Di CesareI de Curtis
Sep 22, 2000·Trends in Cell Biology·M Symons, J Settleman
Nov 4, 2000·The Journal of Cell Biology·T R JacksonP A Randazzo
Mar 27, 2001·EMBO Reports·S PasqualatoJ Cherfils
Sep 5, 2001·The Journal of Biological Chemistry·A ClaingR J Lefkowitz
Sep 7, 2001·Current Opinion in Cell Biology·C E TurnerM C Brown
Nov 10, 2001·Science·I R Vetter, A Wittinghofer
Nov 24, 2001·Trends in Cell Biology·S KuerstenI W Mattaj
Sep 10, 2002·The Journal of Biological Chemistry·Lorraine C Santy
Oct 16, 2002·The Journal of Cell Biology·Jia-Shu YangVictor W Hsu
Jun 18, 2003·The Journal of Cell Biology·Florence NiedergangPhilippe Chavrier
Aug 13, 2003·The Journal of Biological Chemistry·Julie G Donaldson
Nov 11, 2003·Journal of Biochemistry·Hisataka Sabe
Jan 8, 2004·Cellular Signalling·Paul A Randazzo, Dianne S Hirsch
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shigeru HashimotoHisataka Sabe
May 6, 2004·Journal of Cell Science·Eric MaciaMichel Franco
Jul 3, 2004·The Journal of Biological Chemistry·Shigeru HashimotoHisataka Sabe
Jan 18, 2005·Trends in Cell Biology·Crislyn D'Souza-Schorey

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Citations

Sep 10, 2008·The Journal of Biological Chemistry·Stéphanie KleinFrédéric Luton
Sep 21, 2007·Molecular Biology of the Cell·Neil M GoldenbergMel Silverman
Feb 21, 2008·PloS One·Annelise SahinGilles Merlin
Aug 20, 2011·PloS One·Joana FigueiredoRaquel Seruca
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Dominique PadovaniJacqueline Cherfils
Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·Alastair S RobertsonKathryn R Ayscough
Jun 15, 2007·European Journal of Cell Biology·Jacek Jaworski
Jan 23, 2008·Traffic·Iwona I Smaczynska-de RooijKathryn R Ayscough
Oct 5, 2011·Biochimica Et Biophysica Acta·Kristine M MayleDaniel T Kamei
Jun 4, 2015·Nanotoxicology·Julia SchoelermannMihaela Roxana Cimpan
Mar 29, 2011·Current Biology : CB·Guillaume MontagnacPhilippe Chavrier
Feb 13, 2009·Current Biology : CB·Guillaume MontagnacPhilippe Chavrier
Sep 16, 2015·PLoS Pathogens·Qian ShuCarolyn B Coyne
Nov 27, 2015·Journal of Immunology Research·Jouda GamaraSylvain G Bourgoin
Jul 28, 2016·Journal of Cellular Physiology·Gordana Blagojević ZagoracPero Lučin
Dec 7, 2007·Biochemical and Biophysical Research Communications·Atsushi Fujita
Feb 21, 2008·European Journal of Immunology·Henrik G SvenssonColin Watts
Sep 1, 2017·American Journal of Physiology. Renal Physiology·Annika HerlemannMartin Hennenberg
Dec 4, 2019·Life Science Alliance·Avery Van DuzerNaoki Oshimori
Jun 25, 2020·British Journal of Pharmacology·Bingsheng LiMartin Hennenberg
Feb 26, 2015·The Journal of Biological Chemistry·Leonardo E PelletánSilvia A Belmonte
Dec 4, 2013·The Journal of Biological Chemistry·Tim Van AckerFrank Peelman
Jun 27, 2019·Nature Cell Biology·James W ClancyCrislyn D'Souza-Schorey
May 23, 2012·Journal of Cell Science·Eric MaciaMichel Franco
Feb 28, 2020·Nature Communications·Tatsiana KosciukHening Lin
Jun 6, 2009·Journal of Cell Science·Cristina SironiDaniela Talarico
Jul 11, 2021·Free Radical Biology & Medicine·Jouda GamaraSylvain G Bourgoin
May 24, 2006·Biochemical and Biophysical Research Communications·Delfi Krishna, Joseph M Le Doux

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