Role of the buffer in retention and adsorption mechanism of ionic species in reversed-phase liquid chromatography. I. Analytical and overloaded band profiles on Kromasil-C18

Journal of Chromatography. a
Fabrice Gritti, G Guiochon

Abstract

The influence of the pH, the concentration, and the nature of the buffer on the retention and overloading behavior of propranolol (pKa = 9.25) on Kromasil-C18 was studied at 2.75 < pH < 6.75, using four buffers (phosphate, acetate, phthalate, and succinate), at three concentrations, 6, 20, and 60 mM. The propranolol band profiles were recorded for three sample sizes, less than 1 microg and 375 microg (sample less concentrated than the buffer), and 7500 microg (band more concentrated than the buffer). Results showed that the buffer concentration, not its pH, controls the retention time of propranolol, in agreement with the chaotropic model. The retention factor depends also on the nature of the buffer, particularly the valence of the basic anion. At moderate loading, the band profiles are well accounted for by a simple bilangmuir model (no adsorbate-adsorbate interactions) with the monovalent anions H2PO4- (pH 2.75), HOOC-Ph-COO- (pH 2.75), HOOC-CH2-CH2-COO- (pH 4.16) and CH3COO- (pH 4.75), and by a bimoreau model (significant adsorbate-adsorbate interactions) with the bivalent anions OOC-Ph-COO- (pH 4.75), OOC-CH2-CH2-COO- (pH 5.61) and HPO4(2-) (pH 6.75). The isotherm were determined using the inverse method. The results show ...Continue Reading

Citations

Jul 13, 2006·Journal of Separation Science·Hisham Hashem, Thomas Jira
Jul 17, 2009·Journal of Separation Science·Sandra Pous-TorresMaria Celia García-Alvarez-Coque
Jun 19, 2020·International Journal of Molecular Sciences·Jolanta FliegerMałgorzata Tatarczak-Michalewska
Jul 1, 2008·Critical Reviews in Analytical Chemistry·Teresa Cecchi
Dec 10, 2014·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Nov 19, 2013·Journal of Chromatography. a·Jolien Van SchoorsAnn Van Eeckhaut
Dec 15, 2004·Analytical Chemistry·Fabrice Gritti, Georges Guiochon
Jul 1, 2005·Analytical Chemistry·Fabrice Gritti, Georges Guiochon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.