Role of the c-myc and the N-myc proto-oncogenes in the immortalization of neural precursors.

Journal of Neuroscience Research
O BernardP F Bartlett

Abstract

To study the role of c-myc and N-myc in the immortalization of neural precursors, we infected neuroepithelial cells isolated from 10-day-old mouse embryos with a new retrovirus vector, pzen, harboring either the c-myc or the N-myc oncogene. The immortalized cell lines contain high levels of the virally expressed myc protein. The amount of myc proteins correlated with the capacity of the cells to differentiate spontaneously in vitro into neurons and glia; cell lines expressing high levels of myc protein can differentiate spontaneously while cell lines expressing low levels of the myc protein resemble epithelial cells. Addition of acidic or basic fibroblast growth factor enhanced differentiation of most cell lines. Some of the cell lines produced neurotrophic growth factors capable of supporting the growth of other cell lines at low density. There was no significant difference between cell lines immortalized with c-myc or with N-myc. Most of the immortalized cells lines generated from bipotential precursors are capable of differentiating into neurons and glia.

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Citations

Jan 1, 1993·Molecular Neurobiology·M Murphy, P F Bartlett
Aug 27, 1998·In Vitro Cellular & Developmental Biology. Animal·Y NakagaitoT Yoshida
Jan 1, 1994·Pharmacology & Therapeutics·P F BartlettM Murphy
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Sep 25, 2008·Developmental Neurobiology·Hedong LiMartin Grumet
Feb 1, 1996·Molecular Neurobiology·S R Whittemore, E Y Snyder
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