Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export.

Journal of Bacteriology
T MinaminoK Namba

Abstract

For construction of the bacterial flagellum, many of the flagellar proteins are exported into the central channel of the flagellar structure by the flagellar type III protein export apparatus. FlhA and FlhB, which are integral membrane proteins of the export apparatus, form a docking platform for the soluble components of the export apparatus, FliH, FliI, and FliJ. The C-terminal cytoplasmic domain of FlhA (FlhA(C)) is required for protein export, but it is not clear how it works. Here, we analyzed a temperature-sensitive Salmonella enterica mutant, the flhA(G368C) mutant, which has a mutation in the sequence encoding FlhA(C). The G368C mutation did not eliminate the interactions with FliH, FliI, FliJ, and the C-terminal cytoplasmic domain of FlhB, suggesting that the mutation blocks the export process after the FliH-FliI-FliJ-export substrate complex binds to the FlhA-FlhB platform. Limited proteolysis showed that FlhA(C) consists of at least three subdomains, a flexible linker, FlhA(CN), and FlhA(CC), and that FlhA(CN) becomes sensitive to proteolysis by the G368C mutation. Intragenic suppressor mutations were identified in these subdomains and restored flagellar protein export to a considerable degree. However, none of these...Continue Reading

References

Jan 1, 1985·Molecular & General Genetics : MGG·K Kutsukake, T Iino
Feb 1, 1984·Journal of General Microbiology·S YamaguchiT Iino
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jul 14, 2000·Journal of Bacteriology·T MinaminoR M Macnab
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Jun 6, 2003·Molecular Microbiology·Laurent ClaretColin Hughes
Sep 2, 2003·Journal of Bacteriology·Gillian M FraserRobert M Macnab
Apr 3, 2004·Journal of Bacteriology·John S Van ArnamRobert M Macnab
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Tohru Minamino, Keiichi Namba
Jun 20, 2006·Journal of Molecular Biology·Tohru MinaminoKeiichi Namba
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lewis D B EvansColin Hughes
Jan 25, 2008·Nature·Koushik PaulKelly T Hughes
Oct 22, 2008·Molecular BioSystems·Tohru MinaminoKeiichi Namba
Aug 12, 2009·Biochemical and Biophysical Research Communications·Ken-Ichi KazetaniKeiichi Namba
Mar 5, 2010·Molecular Microbiology·Yumiko Saijo-HamanoKeiichi Namba

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Citations

Sep 22, 2011·Nature Communications·Tohru MinaminoKeiichi Namba
Nov 20, 2012·Journal of Bacteriology·Tatsuya IbukiTohru Minamino
Oct 14, 2010·PLoS Genetics·Clive S BarkerFadel A Samatey
Jul 9, 2014·Annual Review of Microbiology·Jorge E GalánSamuel Wagner
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pei-Chung LeeArne Rietsch
Jan 20, 2011·Molecular Microbiology·Hui Li, Victor Sourjik
Jan 24, 2014·Molecular Microbiology·Yusuke V MorimotoTohru Minamino
Mar 5, 2010·Molecular Microbiology·Yumiko Saijo-HamanoKeiichi Namba
Sep 26, 2013·Biochimica Et Biophysica Acta·Tohru Minamino
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nandish KhanraCharalampos G Kalodimos
Apr 8, 2014·Journal of Bacteriology·Lynne S CairnsNicola R Stanley-Wall
May 6, 2010·The Journal of Biological Chemistry·Stanley A Moore, Yunhua Jia
Nov 8, 2016·Frontiers in Cellular and Infection Microbiology·Meztlli O GaytánBertha González-Pedrajo
Jun 1, 2018·BMC Genomics·Behrooz DarbaniIrina Borodina
Feb 13, 2021·Biomolecules·Manuel Halte, Marc Erhardt
Jun 2, 2021·Communications Biology·Yumi InoueTohru Minamino
Aug 28, 2015·Nihon saikingaku zasshi. Japanese journal of bacteriology·Tohru Minamino
Apr 5, 2014·Biochemistry·Xiaowei ZhaoJun Liu

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