Role of the carboxyl terminal stop transfer sequence of UGT1A6 membrane protein in ER targeting and translocation of upstream lumenal domain

FEBS Letters
M OuzzineS Fournel-Gigleux

Abstract

We investigated the role of the stop transfer sequence of human UGT1A6 in ER assembly and enzyme activity. We found that this sequence was able to address and translocate the upstream lumenal domain into microsomal membranes in vitro co- and posttranslationally. The signal activity of this sequence was further demonstrated in HeLa cells by its ability to target and maintain the CD4 protein deleted from both the N-terminal signal peptide and C-terminal transmembrane domain into the ER. We showed that total or partial deletion of the stop transfer sequence of UGT1A6 severely impaired enzyme activity highlighting its importance in both membrane assembly and function.

References

Jan 1, 1994·Annual Review of Cell Biology·P Walter, A E Johnson
Oct 15, 1993·Biochemical and Biophysical Research Communications·M NakaiT Endo
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·S PanznerT A Rapoport
Mar 30, 2001·The Journal of Biological Chemistry·C HölscherB Dobberstein
Jun 25, 2003·The Journal of Cell Biology·Nica BorgeseEmanuela Pedrazzini

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Citations

Jan 14, 2012·Drug Metabolism and Pharmacokinetics·Kenjiro NagaokaShizuo Narimatsu
Oct 8, 2009·Drug Metabolism Reviews·Jacques MagdalouMohamed Ouzzine
Dec 3, 2009·Biochemical Pharmacology·Miki NakajimaTsuyoshi Yokoi
Dec 26, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eric LévesqueChantal Guillemette
May 5, 2020·Glycobiology·Krzysztof MikolajczykMarcin Czerwinski

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