Dec 24, 1997

Role of the central metal ion and ligand charge in the DNA binding and modification by metallosalen complexes

Bioconjugate Chemistry
S S MandalSantanu Bhattacharya

Abstract

Several metal complexes of three different functionalized salen derivatives have been synthesized. The salens differ in terms of the electrostatic character and the location of the charges. The interactions of such complexes with DNA were first investigated in detail by UV-vis absorption titrimetry. It appears that the DNA binding by most of these compounds is primarily due to a combination of electrostatic and other modes of interactions. The melting temperatures of DNA in the presence of various metal complexes were higher than that of the pure DNA. The presence of additional charge on the central metal ion core in the complex, however, alters the nature of binding. Bis-cationic salen complexes containing central Ni(II) or Mn(III) were found to induce DNA strand scission, especially in the presence of co-oxidant as revealed by plasmid DNA cleavage assay and also on the basis of the autoradiogram obtained from their respective high-resolution sequencing gels. Modest base selectivity was observed in the DNA cleavage reactions. Comparisons of the linearized and supercoiled forms of DNA in the metal complex-mediated cleavage reactions reveal that the supercoiled forms are more susceptible to DNA scission. Under suitable condition...Continue Reading

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Citations

Mentioned in this Paper

Titrimetry
Manganese
DNA Modification Process
Derivatives
Alkalescens-Dispar Group
RNA Denaturation
Complex (molecular entity)
Organometallic Compounds
Nickel
Cytokinesis of the Fertilized Ovum

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