Role of the chymotrypsin-like membrane-associated proteinase from Treponema denticola ATCC 35405 in inactivation of bioactive peptides.

Infection and Immunity
P L MäkinenS A Syed

Abstract

The ability of washed whole cells of Treponema denticola ATCC 35405 to hydrolyze (inactivate) substance P, bradykinin, and angiotensin I was studied. Substance P was attacked primarily at the Phe-8-Gly-9 bond by a chymotrypsin-like proteinase (CTLP), at Pro-4-Gln-5 by an endo-acting prolyl oligopeptidase (POPase), and at Gln-5-Gln-6 by an endopeptidase (FALGPA-peptidase). Bradykinin was cleaved at Phe-5-Ser-6 by the FALGPA-peptidase and at Pro-7-Phe-8 by the POPase. Angiotensin I was rapidly converted to angiotensin II by the CTLP, and both angiotensin I and angiotensin II were further hydrolyzed at Pro-7-Phe-8 by the POPase. All these enzymes were assumed to be cell associated and were easily extracted with a mild (0.05 to 0.1%) Triton X-100 treatment. Because it was conceivable that the hydrolysis of substance P at the Phe-8-Gly-9 bond was catalyzed by a CTLP described earlier (V.-J. Uitto, D. Grenier, E. C. S. Chan, and B. C. McBride, Infect. Immun. 56:2717-2722, 1988), the enzyme was purified to homogeneity by means of conventional fast protein liquid chromatography procedures. For kinetic studies, Phe-8(4-nitro)-substance P (NSP) (absorption maximum at 309.2 nm, epsilon = 545 M-1 cm-1) was synthesized to replace substance ...Continue Reading

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Citations

Dec 10, 1999·FEMS Microbiology Letters·K Ishihara, K Okuda
Nov 24, 1999·Journal of Periodontal Research·G RosenM N Sela
Jan 13, 2001·Oral Microbiology and Immunology·E C Chan, R McLaughlin
Nov 18, 2003·Journal of Bacteriology·Frederick F CorreiaFloyd E Dewhirst
Mar 23, 2004·FEMS Microbiology Letters·Kazuyuki IshiharaKatsuji Okuda
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Aug 18, 2010·Periodontology 2000·Kazuyuki Ishihara
Sep 10, 1999·Microbiology and Immunology·K Ishihara, K Okuda
May 21, 2003·Clinical Oral Implants Research·Michael N SelaGraciela Rosen
Jan 10, 2006·Periodontology 2000·Zhimin Feng, Aaron Weinberg
Oct 14, 2010·Journal of Dental Research·S G DashperE C Reynolds
Mar 1, 2012·Journal of Oral Microbiology·J Christopher Fenno
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M N Sela
Dec 12, 2001·Oral Microbiology and Immunology·G RosenM N Sela
Aug 5, 2006·Anaerobe·J C Fenno, B C McBride
Jun 5, 2021·Frontiers in Cellular and Infection Microbiology·M Paula Goetting-MineskyJ Christopher Fenno

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