PMID: 9006016Feb 1, 1997Paper

Role of the citrate pathway in glutamate biosynthesis by Streptococcus mutans

Journal of Bacteriology
D G CvitkovitchA S Bleiweis

Abstract

In work previously reported (J. A. Gutierrez, P. J. Crowley, D. P. Brown, J. D. Hillman, P. Youngman, and A. S. Bleiweis, J. Bacteriol. 178:4166-4175, 1996), a Tn917 transposon-generated mutant of Streptococcus mutans JH1005 unable to synthesize glutamate anaerobically was isolated and the insertion point of the transposon was determined to be in the icd gene encoding isocitrate dehydrogenase (ICDH). The intact icd gene of S. mutans has now been isolated from an S. mutans genomic plasmid library by complementation of an icd mutation in Escherichia coli host strain EB106. Genetic analysis of the complementing plasmid pJG400 revealed an open reading frame (ORF) of 1,182 nucleotides which encoded an enzyme of 393 amino acids with a predicted molecular mass of 43 kDa. The nucleotide sequence contained regions of high (60 to 72%) homology with icd genes from three other bacterial species. Immediately 5' of the icd gene, we discovered an ORF of 1,119 nucleotides in length, designated citZ, encoding a homolog of known citrate synthase genes from other bacteria. This ORF encoded a predicted protein of 372 amino acids with a molecular mass of 43 kDa. Furthermore, plasmid pJG400 was also able to complement a citrate synthase (gltA) mutat...Continue Reading

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Citations

Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D G Cvitkovitch
Jan 4, 2012·FEMS Microbiology Letters·Jennifer KimDennis G Cvitkovitch
Oct 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dragana AjdićJoseph J Ferretti
Aug 17, 2006·Nutritional Neuroscience·A Scinska-BienkowskaP Bienkowski
Mar 10, 2010·Future Microbiology·Robert Matsui, Dennis Cvitkovitch
Jul 7, 2016·Folia Microbiologica·Mingyun LiRichard L Gregory
Oct 7, 2010·Journal of Dental Research·N TakahashiG Mayanagi
Jul 22, 2016·FEBS Letters·Jörg Willenborg, Ralph Goethe
Jul 17, 2015·Microbiology and Immunology·Yuka NorimatsuNobuhiro Takahashi
Sep 15, 2020·Microbial Genomics·Fotis E PsomopoulosChristos A Ouzounis
Nov 17, 2009·Journal of Bacteriology·Kevin P O'RourkeGrace A Spatafora
Apr 17, 2013·The Angle Orthodontist·Alinne Ulbrich Mores RymoviczEdvaldo Antonio Ribeiro Rosa

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