Role of the coat protein-RNA interaction in the life cycle of bacteriophage MS2

Molecular & General Genetics : MGG
D S Peabody

Abstract

The coat protein of the RNA bacteriophage MS2 interacts with viral RNA to translationally repress replicase synthesis. This protein-RNA interaction is also thought to play a role in genome encapsidation. In this study the strength of the interaction was perturbed by constructing a recombinant genome containing a super-repressing coat mutation. Because replicase synthesis is prematurely repressed, the mutant produces plaques about five orders of magnitude less efficiently than wild-type. The few plaques obtained are second-site revertants of the original coat mutation and fall into two categories. Those of the first type contain nucleotide substitutions within the translational operator that reduce or destroy its ability to bind coat protein, showing that this interaction is not necessary for genome encapsidation. Revertants of the second type are double mutants in which one substitution converts the coat initiator AUG to AUA and the other substitutes an A for the G normally present two nucleotides upstream of the coat start codon. The mutation of the coat protein gene AUG to AUA, by itself, reduces coat protein synthesis to a few percent of the wild-type level. The second substitution destabilizes the coat initiator stem-loop a...Continue Reading

Citations

Dec 27, 2005·Nucleic Acids Research·Dalibor BlazekB Matija Peterlin
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Eric C DykemanReidun Twarock
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·H E JohanssonO C Uhlenbeck
Nov 27, 2015·Journal of Molecular Biology·Óttar RolfssonPeter G Stockley
Nov 22, 2011·Bio Systems·Meenakshisundaram KandhaveluAndre Sanches Ribeiro
May 5, 2016·Bacteriophage·Peter G StockleyReidun Twarock
Sep 23, 2006·Journal of Molecular Biology·Sjoerd H E van den WormJan van Duin
Sep 6, 2005·Molecular and Biochemical Parasitology·Huafang ShiElisabetta Ullu
May 20, 2015·Applied Microbiology and Biotechnology·Lei ZhangJinming Li
May 30, 2008·Journal of Molecular Biology·David S PeabodyBryce Chackerian
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yutong SongEckard Wimmer
Nov 21, 2007·Biotechnology and Bioengineering·Bradley C BundyJames R Swartz
Dec 7, 2013·BMC Bioinformatics·Sharif ChowdhuryAndre S Ribeiro
Apr 18, 2001·The Journal of Biological Chemistry·F LimD S Peabody
May 1, 2020·Viruses·Jerri C CaldeiraFederica Cavallo
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Zhicheng CuiJunjie Zhang
Mar 16, 2019·Scientific Reports·Samuel M D OliveiraAndre S Ribeiro
Feb 25, 2017·Nature Communications·Shabih ShakeelReidun Twarock
Aug 26, 2020·Progress in Biophysics and Molecular Biology·Karl Victor Gorzelnik, Junjie Zhang
Jun 22, 2000·Virology·N LicisJ Van Duin
Aug 3, 2021·The Journal of Physical Chemistry. B·Digvijay Lalwani Prakash, Shachi Gosavi
Aug 29, 2021·Pharmaceuticals·David S PeabodyBryce Chackerian

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