PMID: 9422597Jan 9, 1998Paper

Role of the cold-box region in the 5' untranslated region of the cspA mRNA in its transient expression at low temperature in Escherichia coli

Journal of Bacteriology
L FangM Inouye

Abstract

Upon temperature downshift, a group of proteins called cold shock proteins, such as CspA, CspB, and CsdA, are transiently induced in Escherichia coli. However, when the 5' untranslated region (5' UTR) of cspA mRNA is overproduced at low temperature, the expression of cold shock genes is prolonged or derepressed. It has been proposed that this effect is due to highly conserved 11-base sequences designated the "cold box" existing in the 5' UTRs of cspA, cspB, and csdA. Here, we demonstrate that the overproduction of the 5' UTR of not only cspA but also cspB and csdA mRNAs causes derepression of all three genes at the same time. Conversely, when the cold-box region was deleted from the cspA 5' UTR its derepression function was abolished. The amount of mRNA from the chromosomal cspA gene was much higher in cells overproducing the wild-type 5' UTR by means of a plasmid than it was in cells overproducing the cold-box-deleted 5' UTR. The stability of the chromosomal cspA mRNA in cells overproducing the wild-type 5' UTR was almost identical to that in cells overproducing the cold-box-deleted 5' UTR. Therefore, the derepression of cspA caused by overproduction of 5' UTR at the end of the acclimation phase occurs at the level of transcri...Continue Reading

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Citations

Sep 8, 2006·Nucleic Acids Research·Markus ZeebJochen Balbach
Nov 4, 2010·RNA Biology·Sangita Phadtare, Konstantin Severinov
Mar 17, 2012·Nature Reviews. Microbiology·Jens Kortmann, Franz Narberhaus
Apr 25, 2014·Microbiology·Ashish Kumar SinghSisinthy Shivaji
Oct 29, 2005·Extremophiles : Life Under Extreme Conditions·Yoshinori MurataHitoshi Iwahashi
Oct 25, 2001·The Journal of Biological Chemistry·Heike Uhlmann-SchifflerHans Stahl
May 12, 2009·Applied and Environmental Microbiology·Ashish K SinghS Shivaji
Apr 6, 2006·Journal of Bacteriology·Geraldine BresolinThilo M Fuchs
Jan 28, 2017·Nucleic Acids Research·Enrico RennellaBernhard Brutscher
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Jan 9, 2018·Nucleic Acids Research·Carlos J CaballeroAlejandro Toledo-Arana
Apr 3, 2003·FEMS Microbiology Letters·Oscar H Martínez-CostaFrancisco Malpartida
May 14, 1999·Current Opinion in Microbiology·S PhadtareM Inouye
May 3, 2000·International Journal of Food Microbiology·J GuzzoC Diviès
Sep 1, 2008·EcoSal Plus·Sangita Phadtare, Masayori Inouye
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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