Role of the conserved DRY motif on G protein activation of rat angiotensin II receptor type 1A

Biochemical and Biophysical Research Communications
K OhyamaT Inagami

Abstract

To delineate the functional importance of the highly conserved triplet amino acid sequence, Asp-Arg-Tyr (DRY) among G protein-coupled receptors in the second intracellular loop, these residues of rat angiotensin II (Ang II) receptor type 1A (AT(1A)) were changed by alanine or glycine by site-directed mutagenesis. These mutant receptors were stably expressed in CHO-K1 cells, and the binding of Ang II, GTP effect, InsP(3) production, and the acidification of the medium in response to Ang II were determined. The effects of GTPgammaS on Ang II binding in the mutant receptors D125A and D125G were markedly reduced. InsP(3) production of the mutant D125A, D125G, R126A, and R126G was markedly reduced. Extracellular acidification of D125A was not distinguishable from untransfected CHO-K1 cells. Mutant Y127A was able to produce InsP(3) and acidify medium comparable with wild type AT(1A). These results indicate as follows; Asp(125) is essential for intracellular signal transduction involving G protein coupling, Arg(126) is essential for coupling of G(q) protein but not other G proteins, and Tyr(127) is not important for G protein coupling.

References

Jan 1, 1992·DNA and Cell Biology·W C ProbstS C Sealfon
Dec 15, 1992·Biochemical and Biophysical Research Communications·K OhyamaT Inagami
Jan 15, 1992·Biochemical and Biophysical Research Communications·S Chaki, T Inagami
Oct 5, 1990·Science·R R FrankeK P Hofmann
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Sep 20, 1997·The Journal of Biological Chemistry·T SanoT Inagami
May 24, 2000·The Journal of Biological Chemistry·T A BennettE R Prossnitz
Jun 9, 2001·Biochemical and Biophysical Research Communications·A ChenK A Jacobson

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Citations

Oct 25, 2011·Journal of Lipid Research·Ross M PotterLaurence J Miller
Jul 12, 2014·Journal of Molecular Endocrinology·Pitchai Balakumar, Gowraganahalli Jagadeesh
Feb 27, 2007·Regulatory Peptides·Edson L SantosJoão B Pesquero
Jan 24, 2007·Regulatory Peptides·Rosana I ReisClaudio M Costa-Neto
Apr 13, 2007·Physiological Reviews·Laerte OliveiraAntonio C M Paiva
Dec 29, 2006·Molecular Pharmacology·G Enrico RovatiRichard R Neubig
Apr 19, 2005·The Journal of Biological Chemistry·David KalantKatherine Cianflone
May 15, 2008·Chemical Reviews·David E Nichols, Charles D Nichols
Oct 14, 2008·Journal of Molecular and Cellular Cardiology·Mark AplinJakob Lerche Hansen

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