Role of the cyclic adenosine monophosphate-dependent protein kinase in the control of meiotic resumption in bovine oocytes cultured with thecal cell monolayers

Biology of Reproduction
F J RichardM A Sirard

Abstract

It has long been known that intracellular levels of cAMP are implicated in the process of meiotic resumption in bovine oocytes. The present study was undertaken to evaluate the effects of various modulators of cAMP in a coculture system with thecal cell monolayers that have been shown to promote meiotic arrest (germinal vesicle [GV] stage) in bovine oocytes. Ovaries were obtained from a slaughterhouse, and oocytes were collected by puncture of 1- to 5-mm follicles. Cumulus-oocyte complexes (COC) were cultured for 18 h in tissue culture medium 199 supplemented with 10% fetal calf serum and various modulators. When H-89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinole-sulfonamide) , a protein kinase A inhibitor, was used alone, a significant increase (p < 0.05) in the percentage of COC maintained in GV stage was observed. In the coculture system with thecal cell monolayers that maintained oocytes at the GV stage, H-89 significantly (p < 0.05) decreased this percentage. When oocytes were denuded of their cumulus cells, H-89 (50 microM) alone did not significantly increase the percentage of oocytes maintained at the GV stage. In addition, denuded oocytes were not significantly maintained at the GV stage when cocultured with thecal c...Continue Reading

Citations

Mar 25, 2000·Theriogenology·M A SirardM Mayes
Jan 22, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Melanie L Sutton-McDowallJeremy G Thompson
Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Atef Ali, Marc-André Sirard
Sep 20, 2000·Biology of Reproduction·E V SolovyevaA Tsafriri
Aug 22, 2014·Biology of Reproduction·Angus D MacaulayClaude Robert
Nov 21, 2015·Biology of Reproduction·Angus D MacaulayClaude Robert

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