PMID: 6966398Mar 1, 1980Paper

Role of the degradation process in the mitogenic effect of epidermal growth factor

Proceedings of the National Academy of Sciences of the United States of America
N SavionD Gospodarowicz

Abstract

The protease inhibitor leupeptin inhibits the degradation process of (125)I-labeled epidermal growth factor ((125)I-EGF) by cultured bovine granulosa cells. At 80 mug/ml, leupeptin inhibited the appearance of degradation products of (125)I-EGF in the medium by 95% during 1 hr of incubation and by 90% during 24 hr of incubation when the cells were exposed to 5 ng of (125)I-EGF per ml. In contrast, cultures exposed to either saturating (10 ng/ml) or nonsaturating (0.1 ng/ml) concentrations of EGF in the presence of leupeptin (80 mug/ml) exhibited an increase in DNA synthesis that was 70-80% that of cultures exposed to EGF alone. Cultures responded to either EGF or fibroblast growth factor with a logarithmic increase in cell number and, over a period of 8 days, the number of cells increased 10- to 18-fold. Addition of leupeptin did not diminish the growth rate of the cultures. In the presence of leupeptin, (125)I-EGF accumulated within the granulosa cells and was in a form that was precipitable with antiserum against EGF and that comigrated on isoelectric focusing with native (125)I-EGF. That a full mitogenic response can be obtained despite a 90-95% inhibition of EGF degradation at either saturating or nonsaturating concentration...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Das, C F Fox
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pastan
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L B Chen, J M Buchanan
May 1, 1972·Endocrinology·R L ByynyS Cohen

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Citations

Jul 1, 1983·Calcified Tissue International·K W NgT J Martin
Dec 15, 1981·Biochemical and Biophysical Research Communications·Y IwamotoI D Goldfine
Jan 1, 1980·Advances in Enzyme Regulation·M Friedkin, E Rozengurt
Apr 17, 1985·Biochimica Et Biophysica Acta·L M MatrisianB E Magun
Jan 1, 1988·The International Journal of Biochemistry·S TakahashiH Naito
Jul 9, 1981·The New England Journal of Medicine·A C King, P Cuatrecasas
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W K Miskimins, N Shimizu
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A C KingP Cuatrecasas
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D M MoriarityC R Savage
May 5, 1981·Biochimica Et Biophysica Acta·Y YardenJ Schlessinger
May 31, 1982·Biochemical and Biophysical Research Communications·A C King, P Cuatrecasas
Nov 16, 1982·Biochemical and Biophysical Research Communications·H MasudaH Endo
Sep 16, 1982·Biochemical and Biophysical Research Communications·B E MagunJ S Finch
Sep 15, 1982·Analytical Biochemistry·L M MatrisianB E Magun
Jan 1, 1983·CRC Critical Reviews in Biochemistry·J SchlessingerY Yarden
Jul 17, 1980·Nature·C R Hopkins
Sep 1, 1982·Journal of Cellular Physiology·W K Miskimins, N Shimizu
Dec 1, 1981·Journal of Cellular Physiology·M A Shupnik, A H Tashjian
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·A C King, P Cuatrecasas
Nov 1, 1983·The Journal of Pathology·D Gospodarowicz
Feb 11, 1981·Molecular and Cellular Biochemistry·E D Adamson, A R Rees
Sep 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J St Hilaire, A L Jones
Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·C A Browne

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