Role of the EBNA-1 protein in pausing of replication forks in the Epstein-Barr virus genome.

The Journal of Biological Chemistry
O V ErmakovaC L Schildkraut

Abstract

We have previously shown that replication forks stall at a family of repeated sequences (FR) within the Epstein-Barr virus latent origin of replication oriP, both in a small plasmid and in the intact Epstein-Barr virus genome. Each of the 20 repeated sequences within the FR contains a binding site for Epstein-Barr nuclear antigen 1 (EBNA-1), the only viral protein required for latent replication. We showed that the EBNA-1 protein enhances the accumulation of paused replication forks at the FR. In this study, we have investigated a series of truncated EBNA-1 proteins to determine the portion of the EBNA-1 protein that is responsible for pausing of forks at the FR. Two-dimensional agarose gel electrophoresis was performed on the products of in vitro replication reactions in the presence of full-length EBNA-1 or proteins with various deletions to assess the extent of fork pausing at the FR. We conclude that a portion of the DNA binding domain is important for fork pausing. We also present evidence indicating that phosphorylation of the EBNA-1 protein or EBNA-1-truncated derivatives is not essential for pausing. To investigate the mechanism of EBNA-1-mediated pausing of replication forks, we asked whether EBNA-1 could inhibit the D...Continue Reading

References

Mar 1, 1992·Journal of Virology·L Frappier, M O'Donnell
Jan 1, 1992·Annual Review of Microbiology·T M Hill
Sep 1, 1992·Molecular and Cellular Biology·S A Greenfeder, C S Newlon
Dec 1, 1991·Molecular and Cellular Biology·V Dhar, C L Schildkraut
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L Frappier, M O'Donnell
Mar 1, 1991·Journal of Virology·R F AmbinderS D Hayward
Mar 1, 1989·Molecular and Cellular Biology·P J KrysanM P Calos
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E H LeeT Horiuchi
Apr 25, 1988·Nucleic Acids Research·M Polvino-BodnarP A Schaffer
Aug 1, 1985·Molecular and Cellular Biology·B W Stillman, Y Gluzman
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J YatesB Sugden
Oct 1, 1995·Journal of Virology·D MackeyB Sugden
Nov 25, 1994·Nucleic Acids Research·B WiesendangerJ M Sogo
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·M FilutowiczD York
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KaulD Bastia
Aug 19, 1994·Journal of Molecular Biology·M T SmithR G Wake
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, M Nonoyama

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Citations

Mar 31, 1998·Current Opinion in Structural Biology·A M EdwardsL Frappier
Apr 15, 2011·Journal of Virology·Jayaraju Dheekollu, Paul M Lieberman
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Ekaterina V Mirkin, Sergei M Mirkin
Oct 4, 2012·Current Opinion in Virology·Lori Frappier
Jan 1, 2012·Scientifica·Lori Frappier
Nov 7, 2013·Nature Reviews. Microbiology·Paul M Lieberman

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