PMID: 9168146Apr 26, 1997Paper

Role of the Gln/Glu residues of trichocellins A-II/B-II in ion-channel formation in lipid membranes and catecholamine secretion from chromaffin cells

Biochimica Et Biophysica Acta
S WadaT Fujita

Abstract

Trichocellins (TC) A-II and B-II, 20-residue peptaibols isolated from conidia of the fungus Trichoderma viride, have the same sequence except for the residue at position 18. Both TCs were found to form voltage-dependent ion-channels in bilayer lipid membranes (BLM) and to induce catecholamine secretion from bovine adrenal chromaffin cells through Ca2+ influx. TC-A-II (Gln18, neutral) was more effective than TC-B-II (Glu18, charged) for macroscopic current induction in BLMs and for catecholamine secretion from chromaffin cells, suggesting that Glu18 is unfavorable for the ion-channel formation in BLMs and chromaffin cell membranes. Nevertheless, single-channel recordings indicated that TC-B-II forms larger pores with longer open lifetimes than those of TC-A-II. This indicates that the negatively charged carboxyl group of Glu at position 18 stabilizes larger pores. The effects of the negative charge of Glu18 on the activities were confirmed by the use of a TC-B-II analog containing the methyl ester of Glu18.

References

Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Montal, P Mueller
Jan 1, 1984·Biophysical Journal·J E HallG R Marshall
Jan 1, 1982·Bioelectromagnetics·G Schwarz, P Savko
Jan 1, 1993·European Biophysics Journal : EBJ·M S Sansom

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Citations

Sep 26, 2008·Chemistry & Biodiversity·Chanikul ChutrakulJohn F Peberdy
Jul 12, 2005·Acta Microbiologica Et Immunologica Hungarica·A SzekeresCs Vágvölgyi
Sep 28, 2007·Natural Product Reports·Juliana F de S Daniel, Edson Rodrigues Filho
May 19, 2007·Chemistry & Biodiversity·Shun-ichi Wada, Reiko Tanaka
Apr 14, 2010·Journal of Natural Products·Fuhang SongLixin Zhang
Feb 6, 2009·Chemical Research in Toxicology·Alexey G KruglovMirja S Salkinoja-Salonen
Jun 12, 2009·Journal of Natural Products·Jinwei RenWenhan Lin

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