PMID: 9178826May 16, 1997Paper

Role of the Ha-ras gene in the malignant transformation of rat liver oval cells

International Journal of Cancer. Journal International Du Cancer
Pablo SteinbergA Seidel

Abstract

We have shown that the oval cell line OC/CDE 22 can be transformed by the highly carcinogenic fjord-region diol epoxides of benzo[c]phenanthrene. Mutational activation of the ras proto-oncogene family has been proposed to be a critical event in the formation of tumors induced by polycyclic aromatic hydrocarbons. Therefore, we investigated whether in the earlier transformed OC/CDE 22 cells any point mutations were detected in the ras proto-oncogene. The results indicate that the malignant transformation of OC/CDE 22 cells by the 4 stereoisomeric benzo[c]phenanthrene diol epoxides in vitro is independent of activation of the Ha-ras proto-oncogene. In addition, Northern and Western blot analyses revealed no overexpression of the Ha-ras protooncogene in the transformed OC/CDE 22 cell lines. However, transfection of the OC/CDE 22 cells with an activated Ha-ras oncogene malignantly transformed the OC/CDE 22 cells, and the transfected cells served as precursor cells of tumors with a cholangiocellular morphology and phenotype. Our latter finding reinforces the view that OC/CDE 22 cells are committed to the bile duct epithelial cell lineage.

References

Nov 1, 1992·Environmental Health Perspectives·M W AndersonR M Maronpot
Dec 1, 1990·Current Opinion in Cell Biology·N Fausto
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BuchmannM Schwarz
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·D ChakravartiE G Rogan
May 1, 1995·Molecular Carcinogenesis·P Cherpillod, P A Amstad
Oct 1, 1993·The Journal of Investigative Dermatology·B L SchneiderM F Kulesz-Martin
Sep 1, 1996·Toxicology and Applied Pharmacology·C C TravisJ Nicholas
Oct 1, 1958·The Journal of Pathology and Bacteriology·J W WILSON, E H LEDUC
Sep 1, 1944·The Journal of Experimental Medicine·E L Opie

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Citations

Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·L ZenderS W Lowe
May 23, 2000·Molecular Carcinogenesis·C SchlegerP Steinberg
Nov 27, 2014·World Journal of Stem Cells·Lei-Bo Xu, Chao Liu

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