PMID: 9423852Jan 10, 1998Paper

Role of the Haemophilus ducreyi Ton system in internalization of heme from hemoglobin

Infection and Immunity
C ElkinsC E Thomas

Abstract

By cloning into Escherichia coli and construction of isogenic mutants of Haemophilus ducreyi, we showed that the hemoglobin receptor (HgbA) is TonB dependent. An E. coli hemA tonB mutant expressing H. ducreyi hgbA grew on low levels of hemoglobin as a source of heme only when an intact H. ducreyi Ton system plasmid was present. In contrast, growth on heme by the E. coli hemA tonB mutant expressing hgbA was observed only at high concentrations of heme, was TonB independent, and demonstrated that H. ducreyi HgbA was not sufficient to function as a typical TonB-dependent heme receptor in E. coli. Allelic replacement of the wild-type H. ducreyi exbB, exbD, and tonB loci with the exbB, exbD, and tonB deletion resulted in an H. ducreyi isogenic mutant unable to utilize hemoglobin but able to utilize hemin at the same levels as the parent strain to fulfill its heme requirement. This finding confirms the TonB dependence of HgbA-mediated hemoglobin utilization and suggests that uptake of hemin in H. ducreyi is TonB independent. Additionally, the H. ducreyi Ton system mutant synthesized increased amounts of HgbA and other heme-regulated outer membrane proteins, consistent with derepression of these proteins due to lower intracellular hem...Continue Reading

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Citations

Feb 26, 2000·Infection and Immunity·C ElkinsB Olsen
Jun 19, 2012·Molecular Membrane Biology·Phillip E KlebbaSalete M Newton
Jun 4, 2002·DNA and Cell Biology·Igor Stojiljkovic, Donna Perkins-Balding
Jun 5, 2002·FEMS Microbiology Letters·Montserrat BoschJordi Barbé
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Jan 27, 2004·Infection and Immunity·Amanda J BeddekPaul R Langford
Mar 18, 2000·Infection and Immunity·E PradelC Locht
Sep 28, 1998·Journal of Bacteriology·C J LazdunskiH Bénédetti
Apr 4, 2000·Current Opinion in Microbiology·C Wandersman, I Stojiljkovic
Oct 2, 2002·Journal of Clinical Microbiology·Kristine PattersonChristopher Elkins

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