Role of the HPRG Component of Striated Muscle AMP Deaminase in the Stability and Cellular Behaviour of the Enzyme

Biomolecules
F Ronca, A Raggi

Abstract

Multiple muscle-specific isoforms of the Zn2+ metalloenzyme AMP deaminase (AMPD) have been identified based on their biochemical and genetic differences. Our previous observations suggested that the metal binding protein histidine-proline-rich glycoprotein (HPRG) participates in the assembly and maintenance of skeletal muscle AMP deaminase (AMPD1) by acting as a zinc chaperone. The evidence of a role of millimolar-strength phosphate in stabilizing the AMPD-HPRG complex of both AMPD1 and cardiac AMP deaminase (AMPD3) is suggestive of a physiological mutual dependence between the two subunit components with regard to the stability of the two isoforms of striated muscle AMPD. The observed influence of the HPRG content on the catalytic behavior of the two enzymes further strengthens this hypothesis. Based on the preferential localization of HPRG at the sarcomeric I-band and on the presence of a Zn2+ binding motif in the N-terminal regions of fast TnT and of the AMPD1 catalytic subunit, we advance the hypothesis that the Zn binding properties of HPRG could promote the association of AMPD1 to the thin filament.

References

Oct 15, 1975·FEBS Letters·A RaggiG Ronca
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W C Kruckeberg, O P Chilson
May 1, 1979·The Journal of Cell Biology·B AshbyR Bischoff
Dec 7, 1977·Biochemical and Biophysical Research Communications·N OgasawaraM Yoshino
Jun 15, 1978·European Journal of Biochemistry·N OgasawaraT Watanabe
Jan 1, 1978·Methods in Enzymology·C J Coffee
Dec 1, 1978·Archives of Biochemistry and Biophysics·D W Sammons, O P Chilson
Nov 15, 1992·Biochimica Et Biophysica Acta·Y YamadaN Ogasawara
Aug 1, 1992·The American Journal of Physiology·K W RundellR L Terjung
Aug 1, 1992·The American Journal of Physiology·K W RundellR L Terjung
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T MorisakiE W Holmes
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R MarquetantE W Holmes
Mar 15, 1987·The Biochemical Journal·A Raggi, M Ranieri-Raggi
Apr 1, 1985·Biochemical Medicine·W N Fishbein
Oct 15, 1985·The Biochemical Journal·J SpychałaW Makarewicz
Aug 15, 1974·FEBS Letters·N OgasawaraM Yoshino
Jul 25, 1984·Journal of Molecular Biology·J Cooper, J Trinick
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N OgasawaraY Yamada
Jan 1, 1984·The International Journal of Biochemistry·K Kaletha, A Skladanowski
Jan 1, 1984·The International Journal of Biochemistry·N OgasawaraT Watanabe
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J F Koretz, C Frieden
Feb 2, 1982·Biochimica Et Biophysica Acta·N OgasawaraT Asano
Jan 1, 1982·Journal of the Neurological Sciences·D J HayesJ A Morgan-Hughes
Aug 1, 1980·The Biochemical Journal·M Ranieri-Raggi, A Raggi
Dec 15, 1994·Biochemical and Biophysical Research Communications·M GrossE W Holmes
Mar 1, 1995·The Biochemical Journal·D R Janero, C Yarwood
Jul 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T H van KuppeveltR L Sabina
Aug 1, 1993·Archives of Biochemistry and Biophysics·J F KoretzK Wang
Mar 1, 1993·The Biochemical Journal·J K ThakkarD Hreniuk
Apr 21, 1993·Biochimica Et Biophysica Acta·W N FishbeinE W Holmes

❮ Previous
Next ❯

Citations

Oct 1, 2020·International Journal of Sport Nutrition and Exercise Metabolism·Giuseppe Delli PaoliPieter de Lange

❮ Previous
Next ❯

Methods Mentioned

BETA
deamination
biopsies
X-ray
ion exchange chromatography
transmission electron microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.