Role of the human T-cell leukemia virus type 1 PTAP motif in Gag targeting and particle release

Journal of Virology
Irene J DorweilerLouis M Mansky

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) Gag is targeted to the plasma membrane for particle assembly and release. How HTLV-1 Gag targeting occurs is not well understood. The PPPY and PTAP motifs were previously shown to be involved in HTLV-1 particle release with PTAP playing a more subtle role in virus budding. These L domains function through the interaction with host cellular proteins normally involved in multivesicular body (MVB) morphogenesis. The plasma membrane pathway rather than the MVB pathway was found to be the primary pathway for HTLV-1 particle release in HeLa cells. Intriguingly, disruption of the PTAP motif led to a defect in the targeting of Gag from the plasma membrane to CD63-positive MVBs. Particles or particle buds were observed to be associated with MVBs by electron microscopy, implying that Gag targeting to the MVB resulted in particle budding. Blocking clathrin-dependent endocytosis was found not to influence localization of the HTLV-1 Gag PTAP mutant, indicating that Gag did not reach the MVBs through clathrin-dependent endocytosis. Our observations imply that the interaction between Gag and TSG101 is not required for Gag targeting to the MVB. Overexpression of dynamitin p50 increased particle relea...Continue Reading

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Citations

May 14, 2010·Journal of Virology·Holly A SadlerLouis M Mansky
Mar 2, 2013·Journal of Virology·Colleen M Holtz, Louis M Mansky
Jul 3, 2007·Virology Journal·Shuzo UrataJiro Yasuda
Dec 7, 2006·PLoS Biology·Nolwenn JouvenetPaul D Bieniasz
Oct 14, 2011·Viruses·Michael D LairmorePatrick L Green
Jul 21, 2015·Microbiology and Immunology·Chisato Narahara, Jiro Yasuda
Dec 8, 2007·Virology·Benjamin J Chen, Robert A Lamb
Apr 11, 2007·The Journal of Biological Chemistry·Qunyan JinKathleen M Mulder
Jul 27, 2010·Critical Reviews in Biochemistry and Molecular Biology·James H Hurley
Oct 30, 2020·Frontiers in Microbiology·Patrick I MpingaboJiro Yasuda

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