PMID: 11607587Nov 7, 1995Paper

Role of the integument in insect defense: pro-phenol oxidase cascade in the cuticular matrix

Proceedings of the National Academy of Sciences of the United States of America
M Ashida, P T Brey

Abstract

The cuticle of the silkworm Bombyx mori was demonstrated to contain pro-phenol oxidase [zymogen of phenol oxidase (monophenol, L-dopa:oxygen oxidoreductase, EC 1.14.18.1)] and its activating cascade. The activating cascade contained at least one serine proteinase zymogen (latent form of pro-phenol oxidase activating enzyme). When the extracted cascade components were incubated with Ca2+, the latent form of pro-phenol oxidase activating enzyme was itself activated and, in turn, converted through a limited proteolysis of pro-phenol oxidase to phenol oxidase. Immuno-gold localization of prophenol oxidase in the cuticle using a cross-reactive hemolymph anti-pro-phenol oxidase antibody revealed a random distribution of this enzyme in the nonlamellate endocuticle and a specific orderly arrayed pattern along the basal border of the laminae in the lamellate endocuticle of the body wall. Furthermore, prophenol oxidase was randomly distributed in the taenidial cushion of the tracheal cuticle. At the time of pro-phenol oxidase accumulation in the body wall cuticle, no pro-phenol oxidase mRNA could be detected in the epidermal tissue, whereas free-circulating hemocytes contained numerous transcripts of pro-phenol oxidase. Our results sugge...Continue Reading

References

Mar 30, 1990·European Journal of Biochemistry·M AshidaP T Brey
Apr 1, 1981·Cell·L Kalfayan, P C Wensink
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·T KawabataM Ashida
Jun 20, 1995·Archives of Biochemistry and Biophysics·Y YasuharaM Ashida
Jul 1, 1993·Journal of Invertebrate Pathology·L GolkarP T Brey
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P T BreyM Ashida

❮ Previous
Next ❯

Citations

May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A J Nappi, E Ottaviani
Jul 19, 2000·Archives of Biochemistry and Biophysics·M SugumaranK Valivittan
Feb 8, 2008·Journal of Computer-aided Molecular Design·Chao-Bin XueXing-Xiang Gao
Sep 1, 1996·FEMS Immunology and Medical Microbiology·M TsuchiyaS Matsuura
Apr 10, 2013·Bioorganic & Medicinal Chemistry·Shrikant S GawandeChandrahasya N Khobragade
Jun 5, 2004·Developmental and Comparative Immunology·Kathryn NewtonDavid Raftos
Mar 27, 2004·Developmental and Comparative Immunology·Heinz Decker, Elmar Jaenicke
Apr 29, 2005·Insect Biochemistry and Molecular Biology·Anete Pedro LourençoZilá Luz Paulino Simões
Apr 5, 2005·Insect Biochemistry and Molecular Biology·A J Nappi, B M Christensen
Dec 31, 2003·Molecular Immunology·Silvia Naitza, Petros Ligoxygakis
Aug 11, 1998·Immunology Letters·P Vilmos, E Kurucz
Jun 1, 1997·Parasitology Today·Y Carton, A J Nappi
Dec 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E H Richards, J P Edwards
Apr 2, 1998·Current Opinion in Immunology·K Söderhäll, L Cerenius
Jul 19, 2000·Insect Biochemistry and Molecular Biology·M R ChaseM Sugumaran
Apr 9, 2005·Heredity·S A O Armitage, M T Siva-Jothy
Apr 22, 2008·Photochemistry and Photobiology·Antje BrackHeinz Decker
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Oct 9, 2004·Annual Review of Entomology·Paul Schmid-Hempel
Jan 1, 1997·Annual Review of Nutrition·J J Winzerling, J H Law
Jul 23, 2004·PLoS Biology·Michael J Galko, Mark A Krasnow
Apr 16, 1998·Insect Biochemistry and Molecular Biology·D S ParkH Y Park
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·N B TerwilligerM Ryan
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki ArakaneKarl J Kramer
Jul 25, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Mert Olgun KarataşOktay Arslan
Jan 10, 2016·Developmental and Comparative Immunology·Brendon Parsons, Edan Foley
Jul 19, 2015·Journal of Bioscience and Bioengineering·Amrita AnantharamanRamamoorthy Siva
Sep 2, 2009·Developmental and Comparative Immunology·Chuanjie QinErchao Li
Nov 18, 2008·Insect Biochemistry and Molecular Biology·I-Y TsaoC-C Chen
Jun 14, 2008·Insect Biochemistry and Molecular Biology·Thi Thuy An NguyenConrad Cloutier
Jun 3, 2008·Journal of Insect Physiology·Eleanor R HaineJens Rolff

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.