PMID: 5911514Mar 1, 1966

Role of the intestinal brush border in the absorption of cholesterol in rats

The Biochemical Journal
J S DavidJ Ganguly

Abstract

1. Short-term incubation of the everted intestinal sacs of rats in media containing cholesterol oleate or cholesterol plus oleic acid resulted in rapid hydrolysis, but no synthesis, of the sterol ester. 2. On separation of the brush border from the rest of the mucosal cell, almost all of the hydrolytic activity and appreciable amounts of the synthetic activity of the whole cell were found to be present in the brush-border fraction. 3. The isolated brush-border fraction contained considerable amounts of cholesterol, which was always present in the unesterified state; the rest of the cell contained about an equal amount of unesterified cholesterol, but, in addition, small but definite amounts of the esterified sterol were also found in this fraction. 4. On feeding rats with [4-(14)C]cholesterol, which was diluted with 3mg. of cholesterol, it was found that the brush border very rapidly took up the fed sterol without changing its net content of cholesterol. No traces of radioactive cholesterol ester could ever be detected in the isolated brush border after feeding with (14)C-labelled esterified or unesterified cholesterol. 5. The appearance of the labelled sterol was quite rapid in the rest of the cell also, where small proportion...Continue Reading

Citations

Jan 1, 1978·Scandinavian Journal of Gastroenterology·S B Clark, K R Norum
Aug 1, 1969·Journal of the American Geriatrics Society·J S Schweppe, R A Jungmann
Dec 10, 1970·The New England Journal of Medicine·J D GardnerL Laster
Aug 1, 1971·Gut·R Holmes
Aug 1, 1978·The American Journal of Physiology·S B Clark

Related Concepts

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Epicholesterol
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Hydrogen-Ion Concentration
Intestinal Absorption
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