Role of the LTR region between the enhancer and promoter in mink cell focus-forming murine leukemia virus pathogenesis

Virology
Fayth K Yoshimura, T Wang

Abstract

Long terminal repeat (LTR) sequences are important determinants of mink cell focus-forming (MCF) murine leukemia virus pathogenesis. These sequences include the enhancer and sequences between the enhancer and promoter (DEN). In a previous study we showed that a virus missing the DEN region in its LTR was severely attenuated in its ability to induce thymic lymphoma. In this study we observed that a virus with an LTR consisting of DEN but no enhancer sequences was pathogenic. We compared the pathogenicity of this DEN virus with other LTR mutant MCF13 viruses that contained a single enhancer (1R) or a single enhancer plus DEN (1R + DEN). All LTR mutant viruses generated thymic lymphoma, however, at a much lower incidence and with a longer latency compared with wild-type (WT) MCF13 virus. DEN virus replication in the thymus was the lowest compared with the 1R and 1R + DEN viruses. Viral replication in a different thymic subpopulation could not explain the decreased pathogenicity of the LTR mutant viruses compared with WT virus. However, lower levels of mutant virus replication in the thymus compared with WT during the preleukemic period may contribute to the attenuation of pathogenicity. The phenotype of tumors induced by the mutan...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K KawashimaL J Old
Jul 1, 1978·Journal of Virology·R C Nowinski, E F Hays
Jan 1, 1992·Advances in Immunology·E V Rothenberg
Apr 1, 1991·Current Opinion in Immunology·R Scollay
Mar 1, 1991·Journal of Virology·J P StoyeJ M Coffin
Jan 1, 1989·Advances in Immunology·B J Fowlkes, D M Pardoll
Feb 1, 1972·The Journal of Experimental Medicine·W P Rowe, T Pincus
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N Haran-Ghera
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·C S TailorD Kabat
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J L BattiniA D Miller

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.