Role of the MAPK pathway in the observed bystander effect in lymphocytes co-cultured with macrophages irradiated with γ-rays or carbon ions

Life Sciences
Chen DongChunlin Shao

Abstract

The radiation-induced bystander effect (RIBE) has potential implications in cancer risks from space particle radiation; however, the mechanisms underlying RIBE are unclear. The role of the MAPK pathway in the RIBEs of different linear energy transfer (LET) was investigated. Human macrophage U937 cells were irradiated with γ-rays or carbon ions and then co-cultured with nonirradiated HMy2.CIR (HMy) lymphocytes for different periods. The activation of MAPK proteins and the generation of intracellular nitric oxide (NO) and reactive oxygen species (ROS) in the irradiated U937 cells were measured. Micronuclei (MN) formation in the HMy cells was applied to evaluate the bystander damage. Some U937 cells were pretreated with different MAPK inhibitors before irradiation. Additional MN formation was induced in the HMy cells after co-culturing with irradiated U937 cells, and the yield of this bystander MN formation was dependent on the co-culture period with γ-ray irradiation but remained high after 1h of co-culture with carbon irradiation. Further investigations disclosed that the time response of the RIBEs had a relationship with LET, where ERK played a different role from JNK and p38 in regulating RIBEs by regulating the generation of ...Continue Reading

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Citations

Jul 12, 2019·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Igor Shuryak
Apr 3, 2020·Journal of Radiation Research·Qiuning ZhangXiaohu Wang
Jun 24, 2017·Frontiers in Immunology·Sebastian Diegeler, Christine E Hellweg
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ana Rita Thomazela MachadoLusânia Maria Greggi Antunes

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