Role of the Na(+)/K(+)-ATPase beta-subunit in peptide-mediated transdermal drug delivery

Molecular Pharmaceutics
Chang-Li WangLong-Ping Wen

Abstract

In this work, we discovered that the Na(+)/K(+)-ATPase beta-subunit (ATP1B1) on epidermal cells plays a key role in the peptide-mediated transdermal delivery of macromolecular drugs. First, using a yeast two-hybrid assay, we screened candidate proteins that have specific affinity for the short peptide TD1 (ACSSSPSKHCG) identified in our previous work. Then, we verified the specific binding of TD1 to ATP1B1 in yeast and mammalian cells by a pull-down ELISA and an immunoprecipitation assay. Finally, we confirmed that TD1 mainly interacted with the C-terminus of ATP1B1. Our results showed that the interaction between TD1 and ATP1B1 affected not only the expression and localization of ATP1B1, but also the epidermal structure. In addition, this interaction could be antagonized by the exogenous competitor ATP1B1 or be inhibited by ouabain, which results in the decreased delivery of macromolecular drugs across the skin. The discovery of a critical role of ATP1B1 in the peptide-mediated transdermal drug delivery is of great significance for the future development of new transdermal peptide enhancers.

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Citations

Jan 16, 2016·Therapeutic Delivery·Renquan RuanLongping Wen
Jul 29, 2017·Advanced Materials·Rong YangDaniel S Kohane
May 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fan YuWeiping Ding

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