Role of the Neurokinin-1 Receptor in the Promotion of Corneal Epithelial Wound Healing by the Peptides FGLM-NH2 and SSSR in Neurotrophic Keratopathy

Investigative Ophthalmology & Visual Science
Ryoji YanaiKazuhiro Kimura

Abstract

Neurotrophic keratopathy is a corneal epitheliopathy induced by trigeminal denervation that can be treated with eyedrops containing the neuropeptide substance P (or the peptide FGLM-NH2 derived therefrom) and insulin-like growth factor 1 (or the peptide SSSR derived therefrom). Here, we examine the mechanism by which substance P (or FGLM-NH2) promotes corneal epithelial wound healing in a mouse model of neurotrophic keratopathy. The left eye of mice subjected to trigeminal nerve axotomy in the right eye served as a model of neurotrophic keratopathy. Corneal epithelial wound healing was monitored by fluorescein staining and slit-lamp examination. The distribution of substance P, neurokinin-1 receptor (NK-1R), and phosphorylated Akt was examined by immunohistofluorescence analysis. Cytokine and chemokine concentrations in intraocular fluid were measured with a multiplex assay. Topical administration of FGLM-NH2 and SSSR promoted corneal epithelial wound healing in the neurotrophic keratopathy model in a manner sensitive to the NK-1R antagonist L-733,060. Expression of substance P and NK-1R in the superficial layer of the corneal epithelium decreased and increased, respectively, in model mice compared with healthy mice. FGLM-NH2 a...Continue Reading

References

Nov 1, 1990·Peptides·D LortonD L Felten
May 29, 1981·Neuroscience Letters·A MillerI W Chubb
Aug 7, 1981·Neuroscience Letters·K TervoO Eränkö
Apr 29, 1998·The New England Journal of Medicine·A LambiaseL Aloe
Apr 13, 1999·Trends in Neurosciences·M KoltzenburgS B McMahon
Jan 6, 2000·Current Opinion in Ophthalmology·A LambiaseS Bonini
May 30, 2001·The International Journal of Biochemistry & Cell Biology·S Harrison, P Geppetti
Nov 25, 2003·Eye·S BoniniA Lambiase
Feb 25, 2005·Investigative Ophthalmology & Visual Science·Naoyuki YamadaTeruo Nishida
Aug 1, 2006·Investigative Ophthalmology & Visual Science·Naoyuki YamadaTeruo Nishida
Nov 30, 2006·The Ocular Surface·Teruo Nishida
Feb 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hon-Wai KoonCharalabos Pothoulakis
Jun 21, 2008·Investigative Ophthalmology & Visual Science·Zimei ZhouLinda D Hazlett
Feb 24, 2009·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Michael LeeTimothy J Carroll
Jun 23, 2009·Current Opinion in Ophthalmology·Teruo Nishida, Ryoji Yanai
Apr 16, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K J PaunickaJ Y Niederkorn
Jul 22, 2015·Progress in Retinal and Eye Research·Alexander V Ljubimov, Mehrnoosh Saghizadeh
Oct 9, 2015·Cornea·Ryoji YanaiKoh-Hei Sonoda
Nov 1, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Subhash GaddipatiSusmit Suvas
Aug 23, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susmit Suvas
Nov 23, 2017·Frontiers in Immunology·Simmie L FosterSébastien Talbot
Feb 17, 2018·Drugs of Today·M SacchettiA Lambiase
Apr 27, 2018·Progress in Retinal and Eye Research·Harminder S DuaChristophe Baudouin
Sep 22, 2018·Journal of Cellular Physiology·Leonardo MastropasquaHarminder S Dua
Mar 16, 2019·Nature Reviews. Immunology·Pankaj BaralIsaac M Chiu
Aug 3, 2019·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·Stefan MerglerUwe Pleyer
Sep 19, 2019·The Ocular Surface·Antonio Di ZazzoStefano Bonini
Oct 10, 2019·Expert Opinion on Investigational Drugs·Dominique Bremond-GignacFrederic Chiambaretta
Oct 18, 2019·Cell Research·Felipe A Pinho-Ribeiro, Isaac M Chiu
Nov 24, 2019·The Ocular Surface·Sami SaadEric E Gabison
Apr 19, 2020·Investigative Ophthalmology & Visual Science·Sudha Neelam, Jerry Y Niederkorn

❮ Previous
Next ❯

Citations

Jun 8, 2021·Progress in Retinal and Eye Research·Romina Mayra Lasagni VitarGiulio Ferrari

❮ Previous
Next ❯

Methods Mentioned

BETA
scraping
fluorescence imaging
PCR

Software Mentioned

Plex Manager
Bio
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.