PMID: 9190799Jun 1, 1997Paper

Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4

Journal of Bacteriology
M ItoT A Krulwich

Abstract

Application of protoplast transformation and single- and double-crossover mutagenesis protocols to alkaliphilic Bacillus firmus OF4811M (an auxotrophic strain of B. firmus OF4) facilitated the extension of the sequence of the previously cloned nhaC gene, which encodes an Na+/H+ antiporter, and the surrounding region. The nhaC gene is part of a likely 2-gene operon encompassing nhaC and a small gene that was designated nhaS; the operon is preceded by novel direct repeats. The predicted alkaliphile NhaC, based on the extended sequence analysis, would be a membrane protein with 462 amino acid residues and 12 transmembrane segments that is highly homologous to the deduced products of homologous genes of unknown function from Bacillus subtilis and Haemophilus influenzae. The full-length version of nhaC complemented the Na+-sensitive phenotype of an antiporter-deficient mutant strain of Escherichia coli but not the alkali-sensitive growth phenotypes of Na+/H+-deficient mutants of either alkaliphilic B. firmus OF4811M or B. subtilis. Indeed, NhaC has no required role in alkaliphily, inasmuch as the nhaC deletion strain of B. firmus OF4811M, N13, grew well at pH 10.5 at Na+ concentrations equal to or greater than 10 mM. Even at lower N...Continue Reading

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Citations

Dec 5, 2012·Archives of Microbiology·Pedro M F SousaAna M P Melo
Aug 27, 2014·Extremophiles : Life Under Extreme Conditions·Dimitry Y SorokinGerard Muyzer
Jul 1, 2017·Extremophiles : Life Under Extreme Conditions·Ya-Hui ShaoWei-Dong Lu
Oct 17, 2007·FEMS Microbiology Letters·Chong Wai LiewNazalan Najimudin
Apr 2, 2008·Annals of the New York Academy of Sciences·Noha M Mesbah, Juergen Wiegel
Nov 29, 2013·Genome Announcements·David G WernickJames C Liao
Sep 24, 2014·Current Microbiology·Xue YanVianney Pichereau
Mar 23, 2017·Canadian Journal of Microbiology·Yanhong WangJuquan Jiang
Mar 11, 2015·The Journal of Veterinary Medical Science·Mai YamamotoKoichiro Yamamoto
Jul 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masahiro ItoTerry A Krulwich
Mar 15, 2001·Biochimica Et Biophysica Acta·T A KrulwichA A Guffanti
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·K Horikoshi

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