Abstract
To determine if inhibiting neuropilin-1 (NRP-1) affects the radiosensitivity of NSCLC cells through a vascular endothelial growth factor receptor 2 (VEGFR2)-independent pathway, and to assess the underlying mechanisms. The expression of VEGFR2, NRP-1, related signaling molecules, abelson murine leukemia viral oncogene homolog 1 (ABL-1), and RAD51 were determined by RT-PCR and Western blotting, respectively. Radiosensitivity was assessed using the colony-forming assay, and the cell apoptosis were analyzed by flow cytometry. We selected two cell lines with high expression levels of VEGFR2, including Calu-1 cells that have high NRP-1 expression, and H358 cells that have low NRP-1 expression. Upon inhibition of p-VEGFR2 by apatinib in Calu-1 cells, the expression of NRP-1 protein and other related proteins in the pathway was still high. Upon NRP-1 siRNA treatment, the expression of both NRP-1 and RAD51 decreased (p < 0.01; p < 0.05). Upon ABL-1 siRNA treatment, the expression of NRP-1 was increased and the expression of RAD51 was unchanged. Calu-1 cells treated with NRP-1 siRNA exhibited significantly higher apoptosis and radiation sensitivity in radiation therapy compared to Calu-1 cells treated with apatinib alone (p < 0.01; p < ...Continue Reading
References
Nov 15, 2007·PloS One·Ling WangDebabrata Mukhopadhyay
Jun 12, 2008·Carcinogenesis·Jen-Chung KoYun-Wei Lin
Jun 26, 2010·The Journal of Clinical Investigation·Sheng CaoVijay H Shah
Jan 20, 2011·Molecular and Cellular Biology·Ian M EvansPaul Frankel
Nov 22, 2011·International Journal of Radiation Oncology, Biology, Physics·Xiao-Dong JiangJin-Ming Yu
Mar 7, 2012·The Journal of Experimental Medicine·Petra HamerlikJiri Bartek
Mar 13, 2012·Critical Reviews in Oncology/hematology·Peter M Ellis, Khalid Al-Saleh
Jan 15, 2013·Blood·Alessandro FantinChristiana Ruhrberg
Mar 5, 2013·The Journal of Clinical Investigation·Sampurna ChatterjeeRoland T Ullrich
Apr 26, 2013·Cancer Investigation·John D HainsworthF Anthony Greco
Sep 24, 2013·Diagnostic Pathology·Yu XuYao Wang
Nov 7, 2013·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·X JiangL Liu
Jan 1, 2011·Cancers·Camille Grandclement, Christophe Borg
Jan 1, 2014·Clinical Lung Cancer·Xiao-dong JiangLiang Liu
Feb 7, 2014·Radiation Oncology Journal·Seung-Gu Yeo, Eun Seog Kim
May 28, 2014·The Journal of Experimental Medicine·Claudio RaimondiChristiana Ruhrberg
Jul 1, 2014·Pathology·Dipak PanigrahyDiane R Bielenberg
Aug 26, 2014·PloS One·Kemal AlpaySakari Hietanen
Sep 19, 2014·Biochemical Society Transactions·Claudio Raimondi
Jul 7, 2015·Journal of Cellular and Molecular Medicine·Juan Cong DongShun Zi Jin
Oct 16, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y LiuX Jiang
Nov 7, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·L LiuX Jiang
Nov 11, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xing GaoLei Xu
Jan 1, 2015·Molecular & Cellular Oncology·Claudio RaimondiChristiana Ruhrberg
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shyamal SubramanyamMaria Spies
Citations
Apr 28, 2019·International Journal of Molecular Sciences·Virginia Napolitano, Luca Tamagnone
Jul 10, 2019·Cancer Biomarkers : Section a of Disease Markers·Yong-Yao GuZhi-Gang Peng
Feb 14, 2019·Journal of Experimental & Clinical Cancer Research : CR·Lijun LiangXiaodong Jiang
Apr 4, 2020·Journal of Molecular Histology·Ho Seok SeoHan Hong Lee
Aug 9, 2020·Frontiers in Cell and Developmental Biology·Aurore Dumond, Gilles Pagès
May 26, 2021·IUBMB Life·Lianggeng XiongTian Xia
Aug 26, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chunfeng XieCaiyun Zhong
Nov 12, 2021·PeerJ·Lin LiHuawei Zou