Role of the NRP-1-mediated VEGFR2-independent pathway on radiation sensitivity of non-small cell lung cancer cells

Journal of Cancer Research and Clinical Oncology
Chenxi HuXiao-dong Jiang

Abstract

To determine if inhibiting neuropilin-1 (NRP-1) affects the radiosensitivity of NSCLC cells through a vascular endothelial growth factor receptor 2 (VEGFR2)-independent pathway, and to assess the underlying mechanisms. The expression of VEGFR2, NRP-1, related signaling molecules, abelson murine leukemia viral oncogene homolog 1 (ABL-1), and RAD51 were determined by RT-PCR and Western blotting, respectively. Radiosensitivity was assessed using the colony-forming assay, and the cell apoptosis were analyzed by flow cytometry. We selected two cell lines with high expression levels of VEGFR2, including Calu-1 cells that have high NRP-1 expression, and H358 cells that have low NRP-1 expression. Upon inhibition of p-VEGFR2 by apatinib in Calu-1 cells, the expression of NRP-1 protein and other related proteins in the pathway was still high. Upon NRP-1 siRNA treatment, the expression of both NRP-1 and RAD51 decreased (p < 0.01; p < 0.05). Upon ABL-1 siRNA treatment, the expression of NRP-1 was increased and the expression of RAD51 was unchanged. Calu-1 cells treated with NRP-1 siRNA exhibited significantly higher apoptosis and radiation sensitivity in radiation therapy compared to Calu-1 cells treated with apatinib alone (p < 0.01; p < ...Continue Reading

References

Nov 15, 2007·PloS One·Ling WangDebabrata Mukhopadhyay
Nov 22, 2011·International Journal of Radiation Oncology, Biology, Physics·Xiao-Dong JiangJin-Ming Yu
Mar 7, 2012·The Journal of Experimental Medicine·Petra HamerlikJiri Bartek
Mar 13, 2012·Critical Reviews in Oncology/hematology·Peter M Ellis, Khalid Al-Saleh
Mar 5, 2013·The Journal of Clinical Investigation·Sampurna ChatterjeeRoland T Ullrich
Nov 7, 2013·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·X JiangL Liu
Jan 1, 2011·Cancers·Camille Grandclement, Christophe Borg
May 28, 2014·The Journal of Experimental Medicine·Claudio RaimondiChristiana Ruhrberg
Sep 19, 2014·Biochemical Society Transactions·Claudio Raimondi
Jul 7, 2015·Journal of Cellular and Molecular Medicine·Juan Cong DongShun Zi Jin
Oct 16, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y LiuX Jiang
Nov 7, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·L LiuX Jiang
Nov 11, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xing GaoLei Xu
Jan 1, 2015·Molecular & Cellular Oncology·Claudio RaimondiChristiana Ruhrberg
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shyamal SubramanyamMaria Spies

❮ Previous
Next ❯

Citations

Apr 28, 2019·International Journal of Molecular Sciences·Virginia Napolitano, Luca Tamagnone
Jul 10, 2019·Cancer Biomarkers : Section a of Disease Markers·Yong-Yao GuZhi-Gang Peng
Apr 4, 2020·Journal of Molecular Histology·Ho Seok SeoHan Hong Lee
Aug 9, 2020·Frontiers in Cell and Developmental Biology·Aurore Dumond, Gilles Pagès
Aug 26, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chunfeng XieCaiyun Zhong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Related Papers

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
L LiuX Jiang
Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
Y LiuX Jiang
© 2022 Meta ULC. All rights reserved