Role of the plasma membrane calcium adenosine triphosphatase on domoate-induced intracellular acidification in primary cultures of cerebelar granule cells

Journal of Neuroscience Research
Carmen Vale-GonzálezLuis M Botana

Abstract

Changes in intracellular pH (pH(i)) and cytosolic calcium concentration ([Ca(2+)](c)) caused by the glutamate agonist domoate (DOM) were studied in single cultured mouse cerebellar granule cells (CGC) by using the fluorescent probes 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and simultaneous evaluation of cytosolic calcium concentration with the fluorescent dye Fura-2 acetoxymethyl ester (Fura-2 AM). DOM caused a concentration-dependent increase in [Ca(2+)](c) and a concentration-dependent intracellular acidification of CGC. DOM-induced intracellular acidification was completely abolished by the use of Ca(2+)-free medium, suggesting that it was due mostly to an influx of extracellular calcium. The pH(i) decrease caused by DOM was also completely blocked in the presence of the AMPA/kainate receptor antagonist CNQX, indicating that the DOM-induced intracellular acidification was caused by DOM activation of the AMPA/kainate subtype of glutamate receptors. Different mechanisms that could be involved in DOM-induced pH(i) decrease, such as displacement of H(+) by Ca(2+) from a common intracellular binding site, DOM-induced alteration of pH(i) regulation mechanisms, and a possible acidification caus...Continue Reading

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Citations

Oct 3, 2007·Pflügers Archiv : European journal of physiology·Magdalena M SzewczykAshok K Grover
Jan 13, 2011·The Journal of Biological Chemistry·Damon PoburkoNicolas Demaurex
May 4, 2011·World Journal of Biological Chemistry·Jyoti PandeAshok K Grover
Jul 19, 2008·European Journal of Pharmacology·Ahmed F El-YazbiEdwin E Daniel
Nov 1, 2006·Journal of Neuroscience Research·Carmen Vale-GonzálezLuis M Botana
Oct 22, 2010·Environmental Toxicology and Chemistry·Karen H WatanabeIrvin R Schultz
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Sung-Min HwangYong Ho Kim

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