Role of the proline-rich domain of dynamin-2 and its interactions with Src homology 3 domains during endocytosis of the AT1 angiotensin receptor

The Journal of Biological Chemistry
Márta SzaszákLászló Hunyady

Abstract

In nonneural tissues, the dynamin-2 isoform participates in the formation of clathrin-coated vesicles during receptor endocytosis. In this study, the mechanism of dynamin-2 action was explored during endocytosis of the G protein-coupled AT1A angiotensin receptor expressed in Chinese hamster ovary cells. Dynamin-2 molecules with mutant pleckstrin homology domains or deleted proline-rich domains (PRD) exerted dominant negative inhibition on the endocytosis of radiolabeled angiotensin II. However, only the PRD mutation interfered with the localization of the dynamin-2 molecule to clathrin-coated pits and reduced the inhibitory effect of the GTPase-deficient K44A mutant dynamin-2. Green fluorescent protein-tagged Src homology 3 (SH3) domains of endophilin I and amphiphysin II, two major binding partners of dynamins, also inhibited AT1A receptor-mediated endocytosis of angiotensin II. These effects were partially or fully, respectively, restored by the overexpression of dynamin-2. Transient overexpression of these SH3 domains also reduced the localization of dynamin-2 to clathrin-coated pits. These data indicate that, similar to the recruitment of dynamin-1 during the recycling of synaptic vesicles, interaction of the dynamin-2 PRD ...Continue Reading

References

Jan 5, 1996·The Journal of Biological Chemistry·H S ShpetnerR B Vallee
Mar 28, 1997·The Journal of Biological Chemistry·E de HeuvelP S McPherson
Apr 25, 1997·The Journal of Biological Chemistry·P M OkamotoR B Vallee
Aug 1, 1997·Current Biology : CB·P WiggeH T McMahon
Oct 27, 1997·The Journal of Biological Chemistry·K D MichevaP S McPherson
Jan 10, 1998·The EMBO Journal·A B MuhlbergS L Schmid
Mar 7, 1998·The Journal of Biological Chemistry·B BarylkoJ P Albanesi
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·M J Rebecchi, S Scarlata
Aug 28, 1998·Current Opinion in Cell Biology·S L SchmidP De Camilli
Sep 3, 1998·Molecular Biology of the Cell·H CaoM A McNiven
Jan 16, 1999·Journal of the American Society of Nephrology : JASN·S J ElliotG E Striker
Jan 16, 1999·Molecular and Cellular Biology·M AchiriloaieJ P Albanesi
Feb 4, 1999·Regulatory Peptides·W G Thomas
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A LaporteL S Barak
Apr 1, 1999·Annual Review of Physiology·M BünemannM M Hosey
Apr 17, 1999·Cellular Signalling·L WunderlichL Buday
Jul 10, 1999·Science·M Marsh, H T McMahon
Aug 24, 1999·The Journal of Biological Chemistry·J L Whistler, M von Zastrow
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y TangR J Lefkowitz
Dec 10, 1999·The Journal of Biological Chemistry·O KranenburgW H Moolenaar
Dec 22, 1999·The Journal of Experimental Medicine·E S GoldA Aderem
May 10, 2000·The Journal of Biological Chemistry·Y WerbonatC J van Koppen
Jun 29, 2000·Trends in Pharmacological Sciences·T BallaP Várnai
Jul 13, 2000·The Journal of Cell Biology·K N FishH Damke
Sep 1, 2000·Regulatory Peptides·L HunyadyZ Gáborik
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J E Hinshaw
Feb 13, 2001·Molecular Pharmacology·Z GáborikL Hunyady
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T A KohoutR J Lefkowitz

❮ Previous
Next ❯

Citations

Dec 24, 2005·Cell and Tissue Research·Cheryl SavdieThomas Stroh
Apr 3, 2003·Trends in Endocrinology and Metabolism : TEM·Walter G Thomas, Hongwei Qian
Mar 4, 2003·Molecular and Cellular Biology·Hong CaoMark A McNiven
Sep 24, 2010·American Journal of Physiology. Renal Physiology·Haidong HuangGuangping Chen
Aug 11, 2007·Journal of Separation Science·Katrien LanckmansYvette Michotte
Dec 7, 2010·Journal of Cellular and Molecular Medicine·Ivana KnezevicSanda Predescu
Dec 15, 2004·Journal of Pharmacological and Toxicological Methods·K H S ArunP Ramarao
Sep 29, 2004·Journal of Structural Biology·Dganit DaninoJenny E Hinshaw
Oct 13, 2012·Communicative & Integrative Biology·Frank BürmannMarc Bramkamp
Dec 9, 2003·The Journal of Biological Chemistry·Philippe SarretAlain Beaudet
Mar 27, 2004·Physiological Reviews·András Spät, László Hunyady
Nov 10, 2006·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Jan Jozef BraszkoPrzemyslaw Wielgat
Aug 10, 2004·The Journal of Biological Chemistry·Richard Lundmark, Sven R Carlsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.