PMID: 7085754Mar 1, 1982Paper

Role of the reticulum in the stability and shape of the isolated human erythrocyte membrane

The Journal of Cell Biology
Y LangeT L Steck


In order to examine the widely held hypothesis that the reticulum of proteins which covers the cytoplamsic surface of the human erythrocyte membrane controls cell stability and shape, we have assessed some of its properties. The reticulum, freed of the bilayer by extraction with Triton X-100, was found to be mechanically stable at physiological ionic strength but physically unstable at low ionic strength. The reticulum broke down after a characteristic lag period which decreased 500-fold between 0 degrees and 37 degrees C. The release of polypeptide band 4.1 from the reticulum preceded that of spectrin and actin, suggesting that band 4.1 might stabilize the ensemble but is not essential to its integrity. The time-course of breakdown was similar for ghosts, the reticulum inside of ghosts, and the isolated reticulum. However, at very low ionic strength, the reticulum was less stable within the ghost than when free; at higher ionic strength, the reverse was true. Over a wide range of conditions the membrane broke down to vesicles just as the reticulum disintegrated, presumably because the bilayer was mechanically stabilized by this network. The volume of both ghosts and naked reticula varied inversely and reversibly with ionic str...Continue Reading


Jun 1, 1977·The Journal of Cell Biology·W Birchmeier, S J Singer
Dec 14, 1979·The Journal of Membrane Biology·V T Marchesi
Oct 19, 1979·Biochimica Et Biophysica Acta·M P Sheetz
Jan 1, 1977·Journal of Supramolecular Structure·J F Hainfeld, T L Steck
Aug 1, 1978·European Journal of Biochemistry·E Ungewickell, W Gratzer
Jan 28, 1977·The Journal of Membrane Biology·E A Evans, R M Hochmuth
Oct 1, 1976·The Journal of Clinical Investigation·S E LuxM J Karnovsky
Jan 26, 1973·Biochimica Et Biophysica Acta·J AvruchJ R Carter
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu
May 1, 1980·The Journal of Cell Biology·V Fowler, D L Taylor
Aug 1, 1980·The Journal of Cell Biology·R M JohnsonD B Meyer
Dec 31, 1980·Biochemical and Biophysical Research Communications·C M Cohen, C Korsgren
Feb 1, 1981·The Journal of Cell Biology·V P Patel, G Fairbanks


Jan 1, 1991·Cell Motility and the Cytoskeleton·J A UrsittiR J Bloch
Sep 21, 2007·Bulletin of Mathematical Biology·H HägerstrandA Iglic
Jan 1, 1982·The Journal of Membrane Biology·Y LangeT L Steck
Jan 1, 1984·The Journal of Membrane Biology·Y Lange, T L Steck
Jul 24, 1998·Biochimica Et Biophysica Acta·M Rasia, A Bollini
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Seigneuret, P F Devaux
Nov 1, 1985·The Journal of Cell Biology·R R Dubreuil, G B Bouck
Mar 1, 1986·The Journal of Cell Biology·B W ShenT L Steck
Aug 1, 1986·The Journal of Cell Biology·J R Apgar, M F Mescher
Jun 1, 1984·The Journal of Cell Biology·J E Ferrell, W H Huestis
Sep 1, 1984·The Journal of Cell Biology·B W ShenT L Steck
Feb 15, 2002·Current Opinion in Hematology·M Nakao
Dec 1, 1985·Acta Physiologica Scandinavica·G Engström, I B Täljedal
Feb 12, 1993·Science·C F SchmidtG S Grest
Dec 1, 1985·The Journal of Clinical Investigation·J S OwenW Gratzer
Mar 13, 2014·Pflügers Archiv : European journal of physiology·Teresa Tiffert, Virgilio L Lew
Jan 1, 1988·CRC Critical Reviews in Biochemistry·S R GoodmanI S Zagon
Jan 1, 1986·Critical Reviews in Oncology/hematology·S Zail
May 12, 1987·Biochimica Et Biophysica Acta·B IsomaaG Paatero
Jun 1, 1992·Experimental Cell Research·J L WhatmoreJ A Hickman
Aug 22, 1984·Biochimica Et Biophysica Acta·V DresslerJ D Erusalimsky
Jan 1, 1983·Annals of the New York Academy of Sciences·M P Sheetz, E Alhanaty
Apr 21, 1992·Journal of Theoretical Biology·M J Saxton
Apr 14, 2016·Bioelectrochemistry·I T Ivanov, B Paarvanova
Jul 1, 1983·Analytical Biochemistry·P S BeckerS E Lux
Oct 10, 2006·Bioscience Reports·Abhijit ChakrabartiAmitabha Chattopadhyay
Aug 16, 2016·Biomechanics and Modeling in Mechanobiology·Thomas M Fischer
Jun 4, 1985·Biochemistry·J E FerrellW H Huestis
Dec 21, 1987·Journal of Theoretical Biology·M M Kozlov, V S Markin
Sep 30, 2020·The Journal of Membrane Biology·Dipayan Bose, Abhijit Chakrabarti
Jan 1, 1986·European Biophysics Journal : EBJ·B T StokkeA Elgsaeter
Aug 20, 2002·The Journal of Veterinary Medical Science·Yukio YagiYukio Chikayama

Related Concepts

Serum Proteins
Red Cell Ghost
Cell Surface Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.