PMID: 7514591May 1, 1994Paper

Role of the rfe gene in the synthesis of the O8 antigen in Escherichia coli K-12

Journal of Bacteriology
Paul D RickKathleen Barr

Abstract

The Escherichia coli O8 antigen is a mannan composed of the trisaccharide repeat unit -->3)-alpha-Man-(1-->2)-alpha-Man-(1-->2)-alpha-Man-(1--> (K. Reske and K. Jann, Eur. J. Biochem. 67:53-56, 1972), and synthesis of the O8 antigen is rfe dependent (G. Schmidt, H. Mayer, and P. H. Mäkelä, J. Bacteriol. 127:755-762, 1976). The rfe gene has recently been identified as encoding a tunicamycin-sensitive UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (U. Meier-Dieter, K. Barr, R. Starman, L. Hatch, and P. D. Rick, J. Biol. Chem. 267:746-753, 1992). However, the role of rfe in O8 side chain synthesis is not understood. Thus, the role of the rfe gene in the synthesis of the O8 antigen was investigated in an rfbO8+ (rfb genes encoding O8 antigen) derivative of E. coli K-12 mutant possessing a defective phosphoglucose isomerase (pgi). The in vivo synthesis of O8 side chains was inhibited by the antibiotic tunicamycin. In addition, putative lipid carrier-linked O8 side chains accumulated in vivo when lipopolysaccharide outer core synthesis was precluded by growing cells in the absence of exogenously supplied glucose. The lipid carrier-linked O8 antigen was extracted from cells and treated with mild acid in order to relea...Continue Reading

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Citations

Nov 8, 1996·The Journal of Biological Chemistry·W J Keenleyside, C Whitfield
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Jan 23, 2016·Chemical Society Reviews·Qi-Ying HuRoberto Adamo
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Jul 19, 2015·FEMS Microbiology Letters·Akash KumarAnindya S Ghosh
Jul 20, 2018·Nature·Enrique R RojasKerwyn Casey Huang
Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·L Ponoop Prasad Patro, Thenmalarchelvi Rathinavelan
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Mar 13, 2014·FEMS Microbiology Reviews·Sergei KalynychMiroslaw Cygler
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