Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats

Molecular Medicine Reports
Yu WangLichun Zhang

Abstract

Fasudil, which is primarily prescribed to treat cerebral vasospasm, may also inhibit systemic inflammation and prevent sepsis‑induced acute lung injury (ALI) in rats, although the mechanisms remain elusive. The purpose of the present study was to investigate the role of the rhodopsin (Rho)/Rho‑associated protein kinase (ROCK) signaling pathway in the protective effects of fasudil on ALI in septic rats. A total of 60 Wistar rats were pretreated with fasudil (30 mg/kg) through intraperitoneal injections 1 h prior to cecal ligation and puncture. Administration of fasudil led to reductions in polymorphonuclear neutrophil counts, and the protein concentrations of tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 in the bronchoalveolar lavage fluid of rats with sepsis‑induced ALI. The results demonstrated that fasudil decreased sepsis‑induced bacteremia. In addition, fasudil effectively reduced the Evans blue content, wet/dry lung weight ratio, lung injury score, and expression levels of malondialdehyde and myeloperoxidase. However, the superoxide dismutase activity in the lung tissue of the rats was increased. Activated caspase‑3 activity in lung tissue was reduced to 29% by fasudil. Furthermore, the expression of Rho and ROCK1 ...Continue Reading

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Citations

Aug 28, 2021·Cells·Maria Luísa da Silveira Hahmeyer, José Eduardo da Silva-Santos
Aug 27, 2021·Experimental and Therapeutic Medicine·Xinmin ZhaoShinan Nie

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Methods Mentioned

BETA
GTPases
bronchoalveolar lavage
ELISA
Protein Extraction
MDA

Software Mentioned

Image J
SPSS

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