Role of the Saccharomyces cerevisiae Rad51 paralogs in sister chromatid recombination.

Genetics
Amy M MozlinLorraine S Symington

Abstract

Rad51 requires a number of other proteins, including the Rad51 paralogs, for efficient recombination in vivo. Current evidence suggests that the yeast Rad51 paralogs, Rad55 and Rad57, are important in formation or stabilization of the Rad51 nucleoprotein filament. To gain further insights into the function of the Rad51 paralogs, reporters were designed to measure spontaneous or double-strand break (DSB)-induced sister or nonsister recombination. Spontaneous sister chromatid recombination (SCR) was reduced 6000-fold in the rad57 mutant, significantly more than in the rad51 mutant. Although the DSB-induced recombination defect of rad57 was suppressed by overexpression of Rad51, elevated temperature, or expression of both mating-type alleles, the rad57 defect in spontaneous SCR was not strongly suppressed by these same factors. In addition, the UV sensitivity of the rad57 mutant was not strongly suppressed by MAT heterozygosity, even though Rad51 foci were restored under these conditions. This lack of suppression suggests that Rad55 and Rad57 have different roles in the recombinational repair of stalled replication forks compared with DSB repair. Furthermore, these data suggest that most spontaneous SCR initiates from single-stran...Continue Reading

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Citations

Sep 23, 2008·Nature·Eleni P Mimitou, Lorraine S Symington
Sep 6, 2011·Nature Structural & Molecular Biology·Marcel HohlJohn H J Petrini
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Phoebe S Lee, Thomas D Petes
Apr 3, 2012·Nucleic Acids Research·Lumir KrejciXiaolan Zhao
Aug 31, 2011·Molecular and Cellular Biology·Steven S FosterJohn H J Petrini
Jul 16, 2010·PLoS Genetics·Vanessa A Marrero, Lorraine S Symington
Nov 28, 2013·PLoS Genetics·Heïdi SerraCharles I White
Sep 10, 2014·Nature Structural & Molecular Biology·Michele GiannattasioDana Branzei
Jun 10, 2009·The Journal of Cell Biology·Rebecca C BurgessRodney Rothstein
Jan 23, 2015·Critical Reviews in Biochemistry and Molecular Biology·Karen UsdinCatherine H Freudenreich
May 24, 2011·Critical Reviews in Biochemistry and Molecular Biology·Jie LiuScott W Morrical
Feb 15, 2011·DNA Repair·Christina Mott, Lorraine S Symington
Mar 24, 2016·Nucleic Acids Research·Ryan JankeWolf-Dietrich Heyer
Aug 14, 2012·Molecular Cell·Mireille Tittel-ElmerJennifer A Cobb
Jun 15, 2016·Nucleic Acids Research·Stephen K GodinKara A Bernstein
Sep 4, 2016·FEMS Yeast Research·Julieta Martino, Kara A Bernstein
Dec 24, 2016·Cell Reports·Patrick RuffLorraine S Symington
Nov 9, 2014·Genetics·Lorraine S SymingtonMichael Lisby
Nov 14, 2020·ELife·Shanaya Shital ShahWolf-Dietrich Heyer
Jun 24, 2017·DNA Repair·Erica J PolleysCatherine H Freudenreich

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