Role of the scaffolding protein in P22 procapsid size determination suggested by T = 4 and T = 7 procapsid structures

Biophysical Journal
P A Thuman-CommikeW Chiu

Abstract

Assembly of bacteriophage P22 procapsids requires the participation of approximately 300 molecules of scaffolding protein in addition to the 420 coat protein subunits. In the absence of the scaffolding, the P22 coat protein can assemble both wild-type-size and smaller size closed capsids. Both sizes of procapsid assembled in the absence of the scaffolding protein have been studied by electron cryomicroscopy. These structural studies show that the larger capsids have T = 7 icosahedral lattices and appear the same as wild-type procapsids. The smaller capsids possess T = 4 icosahedral symmetry. The two procapsids consist of very similar penton and hexon clusters, except for an increased curvature present in the T = 4 hexon. In particular, the pronounced skewing of the hexons is conserved in both sizes of capsid. The T = 7 procapsid has a local non-icosahedral twofold axis in the center of the hexon and thus contains four unique quasi-equivalent coat protein conformations that are the same as those in the T = 4 procapsid. Models of how the scaffolding protein may direct these four coat subunit types into a T = 7 rather than a T = 4 procapsid are presented.

References

Nov 28, 1991·Nature·R C LiddingtonS C Harrison
Jun 20, 1990·European Journal of Biochemistry·E Kellenberger
Jan 1, 1989·Annual Review of Biochemistry·M G Rossmann, J E Johnson
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Aug 1, 1988·Journal of Electron Microscopy Technique·M Radermacher
Jul 5, 1988·Journal of Molecular Biology·C Bazinet, J King
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A Crowther
Sep 13, 1974·Nature·J King, S Casjens
Jan 1, 1974·Journal of Supramolecular Structure·S Casjens, J King
Sep 1, 1980·Cell·W C Earnshaw, S R Casjens
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Oct 13, 1995·Journal of Molecular Biology·J F ConwayA C Steven
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·B BergerJ King
Oct 20, 1993·Journal of Molecular Biology·T Dokland, H Murialdo
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E Johnson
Jan 1, 1996·Journal of Structural Biology·Z H ZhouW Chiu
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·D L CASPAR, A KLUG
Nov 23, 1978·Nature·S C HarrisonG Bricogne
Jul 3, 1980·Nature·C Abad-ZapateroT Tsukihara

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Citations

Apr 8, 2000·Current Opinion in Structural Biology·J E Johnson, W Chiu
Jun 6, 2000·Micron : the International Research and Review Journal for Microscopy·P A Thuman-Commike, W Chiu
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Antoni LuqueDavid Reguera
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dong-Hua ChenWah Chiu
Oct 29, 2000·Journal of Bacteriology·C Vander Byl, A M Kropinski
Jun 25, 2010·Journal of Virology·Alexis HuetPascale Boulanger
Jan 4, 2013·Journal of Virology·Maija K PietiläRoger W Hendrix
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A KrolP Ahlquist
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z H ZhouF J Rixon
Oct 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Roya ZandiJoseph Rudnick
Mar 13, 2012·Annual Review of Biophysics·David Veesler, John E Johnson
Oct 8, 2005·The EMBO Journal·Xabier AgirrezabalaJosé L Carrascosa
Mar 30, 2016·Journal of the American Society for Mass Spectrometry·David Z KeiferMartin F Jarrold
Oct 28, 2006·Journal of Molecular Biology·Kristin N ParentCarolyn M Teschke
Sep 15, 2006·Journal of Molecular Biology·Adam S OliaGino Cingolani
Feb 27, 1999·Current Opinion in Structural Biology·L Liljas
Feb 3, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rindia M PutriMelissa S T Koay
May 5, 1999·Current Biology : CB·W R Wikoff, J E Johnson
Nov 25, 1998·Biophysical Journal·R SchwartzB Berger
Dec 7, 2011·Physical Chemistry Chemical Physics : PCCP·Antonio SiberRudolf Podgornik
Apr 27, 2016·The Journal of Physical Chemistry. B·Maria Aznar, David Reguera
Jun 28, 2008·Journal of Molecular Biology·Roman TumaPeter E Prevelige
Aug 11, 2010·The Journal of Cell Biology·James R R Whittle, Thomas U Schwartz
Dec 15, 2016·Scientific Reports·Shakhinur Islam MondalTetsuya Hayashi
Mar 3, 2018·Chemical Society Reviews·William M AumillerTrevor Douglas

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