Role of the stress-activated/c-Jun NH2-terminal protein kinase pathway in the cellular response to adriamycin and other chemotherapeutic drugs.

The Journal of Biological Chemistry
M T Osborn, T C Chambers

Abstract

c-Jun NH2-terminal protein kinase (JNK), a member of the mitogen-activated protein kinase family, is activated in response to many stressful stimuli including heat shock, UV irradiation, protein synthesis inhibitors, and inflammatory cytokines. In this study, we investigated whether JNK plays a role in the cellular response to different drugs commonly used in cancer chemotherapy. Treatment of human KB-3 carcinoma cells with Adriamycin resulted in a time- and dose-dependent activation of JNK of up to 40-fold. Treatment with vinblastine or etoposide (VP-16) also activated JNK, with maximum increases of 6.5- and 4.3-fold, respectively. Consistent with these findings, increased c-Jun phosphorylation was observed after drug treatment of cells. In contrast, none of the drugs significantly activated the extracellular response kinase/mitogen-activated protein kinase pathway. Since these drugs are transport substrates for the MDR1 gene product, P-glycoprotein, JNK was assayed in two multidrug-resistant (MDR) KB cell lines, KB-A1 and KB-V1, selected for resistance to Adriamycin and vinblastine, respectively. Relative to KB-3 cells, basal JNK activity was increased 7-fold in KB-A1 cells and 4-fold in KB-V1 cells, with no change in JNK pro...Continue Reading

References

Jun 1, 1995·Cancer Metastasis Reviews·J M Lee, A Bernstein
Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Aug 3, 1993·Biochemical Pharmacology·A Skladanowski, J Konopa
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Aug 5, 1994·Science·Z Galcheva-GargovaR J Davis
Apr 21, 1993·Journal of the National Cancer Institute·P M Chaudhary, I B Roninson
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
May 12, 1994·Nature·J M KyriakisJ R Woodgett

❮ Previous
Next ❯

Citations

Sep 25, 2004·International Journal of Cancer. Journal International Du Cancer·Maria WartenbergHeinrich Sauer
Jan 1, 1993·Cytotechnology·U A Germann
Feb 3, 2012·Translational Stroke Research·Adam ChodobskiJoanna Szmydynger-Chodobska
Feb 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·Gautam Sethi, Ajit Sodhi
Sep 6, 2002·Biochemical Pharmacology·Edward D Owuor, Ah-Ng Tony Kong
Aug 17, 2001·Mutation Research·A N KongS Mandlekar
Jun 5, 2002·Mechanisms of Ageing and Development·Yousin Suh
Jun 1, 2000·Advances in Enzyme Regulation·P AgostinisP De Witte
Sep 28, 1998·Biochimica Et Biophysica Acta·S H Kaufmann
Sep 2, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·T MinkoJ Kopecek
Sep 5, 2001·Brain Research. Molecular Brain Research·S A ParkK S Choi
Jul 18, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·M BarancíkA Breier
Oct 30, 1998·Critical Reviews in Oncology/hematology·M ClynesK Scanlon
Nov 13, 2008·Cytotechnology·U A Germann, T C Chambers
Nov 13, 2008·Cytotechnology·K W Scotto, D A Egan
Nov 13, 2008·Cytotechnology·T MashimaT Tsuruo
Nov 13, 2008·Cytotechnology·M G RumsbyJ R Warr
Nov 26, 1999·Kidney International·A J IngramJ W Scholey
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrew OberstGerry Melino
Apr 23, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R K DomanN J Donato
May 9, 2006·Antioxidants & Redox Signaling·Yousin Suh, Jan Vijg
Aug 29, 2007·Acta Pharmacologica Sinica·Ki Han KwonAh-Ng Tony Kong
Jan 23, 1999·The Journal of Clinical Investigation·M IgarashiG L King
Dec 2, 2008·Biological Chemistry·Degejing DeYipeng Qi
Oct 17, 2007·Journal of Atherosclerosis and Thrombosis·Masahiko IgarashiMakoto Tominaga
Jul 29, 2000·Biochemical and Biophysical Research Communications·T MashimoK Watabe
Nov 10, 2009·Biological Trace Element Research·Saloua LassouedJos Van Pelt
May 24, 2005·FEBS Letters·Maria BerndtssonAleksandra Mandic Havelka

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.