Role of the Tet38 Efflux Pump in Staphylococcus aureus Internalization and Survival in Epithelial Cells

Infection and Immunity
Q C Truong-BolducDavid C Hooper

Abstract

We previously identified the protein Tet38 as a chromosomally encoded efflux pump of Staphylococcus aureus that confers resistance to tetracycline and certain unsaturated fatty acids. Tet38 also contributes to mouse skin colonization. In this study, we discovered a novel regulator of tet38, named tetracycline regulator 21 (TetR21), that bound specifically to the tet38 promoter and repressed pump expression. A ΔtetR21 mutant showed a 5-fold increase in tet38 transcripts and an 8-fold increase in resistance to tetracycline and fatty acids. The global regulator MgrA bound to the tetR21 promoter and indirectly repressed the expression of tet38. To further assess the full role of Tet38 in S. aureus adaptability, we tested its effect on host cell invasion using A549 (lung) and HMEC-1 (heart) cell lines. We used S. aureus RN6390, its Δtet38, ΔtetR21, and ΔmgrA mutants, and a Δtet38 ΔtetR21 double mutant. After 2 h of contact, the Δtet38 mutant was internalized in 6-fold-lower numbers than RN6390 in A549 and HMEC-1 cells, and the ΔtetR21 mutant was internalized in 2-fold-higher numbers than RN6390. A slight increase of 1.5-fold in internalization was found for the ΔmgrA mutant. The growth patterns of RN6390 and the ΔmgrA and ΔtetR21 mu...Continue Reading

References

Jul 3, 2002·The Journal of Experimental Medicine·Yoichi HirakataDavid P Speert
Dec 21, 2002·The Journal of Antimicrobial Chemotherapy·M A Webber, L J V Piddock
May 6, 2003·Journal of Bacteriology·Que Chi Truong-BolducDavid C Hooper
Jun 7, 2003·Molecular Microbiology·S S IngavaleA L Cheung
Feb 10, 2004·Applied and Environmental Microbiology·Antje BurseMatthias S Ullrich
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Jun LinQijing Zhang
Mar 19, 2005·Journal of Bacteriology·Q C Truong-BolducD C Hooper
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Feb 24, 2006·Antimicrobial Agents and Chemotherapy·Que Chi Truong-BolducDavid C Hooper
Apr 15, 2006·Clinical Microbiology Reviews·Laura J V Piddock
Oct 13, 2006·Current Opinion in Infectious Diseases·H Nikaido, H I Zgurskaya
Sep 5, 2008·Current Protocols in Microbiology·Ambrose L Cheung, Kenneth W Bayles
Nov 26, 2008·Biochimica Et Biophysica Acta·Hiroshi Nikaido, Yumiko Takatsuka
Mar 18, 2010·Journal of Bacteriology·Que Chi Truong-Bolduc, David C Hooper
Aug 10, 2013·The Journal of Antimicrobial Chemotherapy·Yee Gyung KwakDavid C Hooper
Nov 28, 2013·The Journal of Infectious Diseases·Que Chi Truong-BolducDavid C Hooper
Jan 1, 2013·International Journal of Biotechnology for Wellness Industries·Valérie Duval, Ida M Lister

❮ Previous
Next ❯

Citations

Jun 13, 2018·Antimicrobial Agents and Chemotherapy·Q C Truong-BolducD C Hooper
Jun 7, 2017·Antimicrobial Agents and Chemotherapy·Q C Truong-BolducD C Hooper
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Giampiero PietrocolaPietro Speziale
May 26, 2020·BMC Microbiology·Magna Coroa LimaMaria Aparecida Scatamburlo Moreira
Mar 11, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Takumi SatoShigeru Fujimura
Aug 13, 2021·Frontiers in Molecular Biosciences·Martina PasquaBianca Colonna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.