Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell-specific protein 1-associated protein X-1-induced survival, migration and proliferation

Oncology Letters
Bing QianRong Shen

Abstract

The haematopoietic cell-specific protein 1-associated protein X-1 (HAX-1), as a mitochondrial membrane protein, induces cancer progression and metastasis. The present study aimed to investigate the role of HAX-1-induced survival, migration and proliferation of human cervical squamous carcinoma cells and to elucidate its potential molecular mechanisms. The level of HAX-1 was examined by quantitative polymerase chain reaction and western blot analyses. The survival, migration and proliferation of the human cervical squamous carcinoma SiHa cell line were measured by the water-soluble tetrazolium salt (WST-1) assay, Transwell assay and 3H-thymidine incorporation into DNA (3H-TdR) assay, respectively. The intracellular reactive oxygen species (ROS) was estimated by the fluorescence of H2DCFDA, and the mitochondrial membrane potential was tested using a JC-1 probe. The expression of the HAX-1 gene was significantly increased in human cervical carcinoma tissues relative to non-cancerous cervix tissues. Overexpression of HAX-1 increased the survival, migration and proliferation ability of SiHa cells, decreased the production of ROS, and maintained the integrity of the mitochondrial membrane and morphology. The effect brought on these c...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 4, 2010·BMC Cancer·Alicja TrebinskaEwa A Grzybowska
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Jul 16, 2015·Molecular and Cellular Biochemistry·Xueming TanDongming Su
Nov 26, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guoqiang AiG Jayarama Bhat
Feb 27, 2016·Biochemical and Biophysical Research Communications·Katsuya HirasakaTakeshi Nikawa
Sep 14, 2016·The Journal of Experimental Medicine·Alex R D DelbridgeAndreas Strasser
Jun 14, 2017·Therapeutic Advances in Medical Oncology·Edith Borcoman, Christophe Le Tourneau
Jun 16, 2017·Cancer Cell International·Ai-Hua ChenManzhen Zuo
Jul 1, 2017·BMC Gastroenterology·R RosaniaP Malfertheiner
Oct 20, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Mengying WangHan Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.