Role of thioredoxins in the response of Saccharomyces cerevisiae to oxidative stress induced by hydroperoxides

Molecular Microbiology
Ester Ocón Garrido, C M Grant

Abstract

Glutaredoxins and thioredoxins are highly conserved, small, heat-stable oxidoreductases. The yeast Saccharomyces cerevisiae contains two gene pairs encoding cytoplasmic glutaredoxins (GRX1, GRX2) and thioredoxins (TRX1, TRX2), and we have used multiple mutants to determine their roles in mediating resistance to oxidative stress caused by hydroperoxides. Our data indicate that TRX2 plays the predominant role, as mutants lacking TRX2 are hypersensitive, and mutants containing TRX2 are resistant to these oxidants. However, the requirement for TRX2 is only apparent during stationary phase growth, and we present three lines of evidence that the thioredoxin isoenzymes actually have redundant activities as antioxidants. First, the trx1 and trx2 mutants show wild-type resistance to hydroperoxide during exponential phase growth; secondly, overexpression of either TRX1 or TRX2 leads to increased resistance to hydroperoxides; and, thirdly, both Trx1 and Trx2 are equally able to act as cofactors for the thioredoxin peroxidase, Tsa1. The antioxidant activity of thioredoxins is required for both the survival of yeast cells as well as protection against oxidative stress during stationary phase growth, and correlates with an increase in the ex...Continue Reading

References

Jun 1, 1992·Archives of Biochemistry and Biophysics·C CoanR J Mehlhorn
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 1, 1989·Free Radical Biology & Medicine·N J Schisler, S M Singh
May 10, 1995·Archives of Biochemistry and Biophysics·J A ThomasR Honzatko
Jan 1, 1993·Free Radical Research Communications·J M Gutteridge
Jun 1, 1993·Microbiological Reviews·M Werner-WashburneR A Singer
Mar 1, 1996·Molecular Microbiology·M Werner-WashburneV M Peck
Feb 3, 1999·Annual Review of Genetics·A Rietsch, J Beckwith
Mar 11, 1999·Current Opinion in Genetics & Development·W M Toone, N Jones
Mar 13, 1999·The Journal of Biological Chemistry·C H LilligA Holmgren
May 29, 1999·The Journal of Biological Chemistry·J LeeM B Toledano
Jul 3, 1999·The Journal of Biological Chemistry·L VerdoucqY Meyer
Feb 22, 2000·The Journal of Biological Chemistry·S G ParkI H Kim
Apr 8, 2000·Free Radical Biology & Medicine·W JakubowskiG Bartosz
Nov 23, 2000·Mechanisms of Ageing and Development·H Gershon, D Gershon

❮ Previous
Next ❯

Citations

Oct 7, 2008·Applied Microbiology and Biotechnology·Roberto Pérez-TorradoEmilia Matallana
Dec 6, 2011·Journal of Bioenergetics and Biomembranes·Tat'yana TrendelevaRenata Zvyagilskaya
Nov 30, 2002·Current Opinion in Microbiology·Paul K Herman
Aug 12, 2010·Biochemistry·Stefano M MarinoVadim N Gladyshev
Nov 29, 2005·Cell Death and Differentiation·S AkterinA Cedazo-Minguez
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Theodora C SideriChris M Grant
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dmitri E FomenkoVadim N Gladyshev
Aug 13, 2011·The Journal of Biological Chemistry·Theodora C SideriChris M Grant
Mar 11, 2003·Antioxidants & Redox Signaling·Nicolas RouhierJean-Pierre Jacquot
Apr 26, 2012·Antioxidants & Redox Signaling·Yves MeyerChristophe Riondet
Dec 4, 2012·Antioxidants & Redox Signaling·Michel B ToledanoAeid Igbaria
Oct 28, 2005·Molecular Biology of the Cell·Jonathan D Rand, Chris M Grant
Dec 29, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Huali ZhangIvor J Benjamin
Apr 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yaru ZhangYuxing Chen
Aug 4, 2010·Molecular and Cellular Biology·Alessandra da Silva DantasJanet Quinn
Jan 16, 2010·BMC Biochemistry·Darren GreethamChris M Grant
Jan 5, 2012·Microbial Cell Factories·Giusy Manuela AdamoMarina Lotti
Jan 3, 2012·Genetics·Kevin A MoranoW Scott Moye-Rowley
Jul 2, 2014·Molecular Plant-microbe Interactions : MPMI·Anne ViefhuesPaul Tudzynski
Jan 8, 2008·Biochimica Et Biophysica Acta·Enrique HerreroElisa Cabiscol
Sep 25, 2004·Molecular Microbiology·Sylke Müller
Mar 23, 2004·Physiologia Plantarum·Glen L. Wheeler, Chris M. Grant
Oct 18, 2006·Proteins·Rui BaoCong-Zhao Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.