PMID: 2104606Jan 1, 1990Paper

Role of threonine residue 154 in ligand recognition of the tar chemoreceptor in Escherichia coli

Journal of Bacteriology
L Lee, Y Imae

Abstract

The Tar chemoreceptor of Escherichia coli mediates attractant responses to aspartate, maltose, and phenol, repellent responses to Ni2+ and Co2+, and thermoresponses. To understand the role of threonine residue 154, which is located in the ligand-binding domain of Tar, we replaced the residue with serine, isoleucine, and proline by site-directed mutagenesis. The replacements caused reductions in aspartate sensing but had only a small effect on maltose sensing and almost no effect on phenol sensing, repellent sensing, and thermosensing. These results indicate that Thr-154 of Tar is rather specifically involved in aspartate sensing. The reductions in the response threshold for aspartate by the replacements with serine, isoleucine, and proline were less than 1, about 2, and more than 5 orders of magnitude, respectively. When the corresponding threonine residue in the Tsr chemoreceptor was replaced with the same amino acids, roughly similar reductions in the response threshold for serine resulted. Thus, these threonine residues seem to have a common role in detecting the aspartate and serine attractant families. A mechanism by which these chemoreceptors detect the amino acid attractants is discussed.

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Citations

Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shuangyu BiLuhua Lai
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Motohiro HommaIkuro Kawagishi
Apr 26, 2006·The Journal of Biological Chemistry·Tomonori IwamaIkuro Kawagishi
Oct 13, 1995·The Journal of Biological Chemistry·S A Chervitz, J J Falke
Nov 7, 2014·Cellular and Molecular Life Sciences : CMLS·Shuangyu Bi, Luhua Lai
Jun 19, 2007·Journal of Bacteriology·Rong Gao, David G Lynn
Feb 7, 2018·Frontiers in Bioengineering and Biotechnology·Takashi SagawaHiroaki Kojima
Apr 1, 1993·Protein Science : a Publication of the Protein Society·C J Jeffery, D E Koshland
Feb 1, 1991·Journal of Bacteriology·T NaraY Imae
Jun 4, 1993·Cell·J S Parkinson
Dec 20, 2005·Journal of Molecular Biology·Paige DerrMark Goulian
Dec 1, 1993·Microbiological Reviews·M Riley

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